公开(公告)号：US10947494B2

公开(公告)日：2021-03-16

申请号：US15775172

申请日：2016-11-10

Applicant: Pfizer Inc.

Inventor： Sunil Gururao Desai ,  Michael Allen Hanson ,  Jonathan Patrick Kinross ,  Daniel R. Lasko ,  Scott Ellis Lomberk ,  Jason Arnold Lotvin ,  Sujata Kaushikbhai Patel-Brown ,  Weiqiang Sun ,  Peter Anthony Tomasello

IPC: C12N1/20 ,  C12P19/04 ,  C12R1/46

Abstract: The present invention relates to media and fermentation methods for producing polysaccharides in bacterial cell culture. In one aspect, the invention relates to a complex culture medium comprising a vegetable hydrolysate, a yeast extract, and a carbon source. In another aspect, the invention relates to a defined media having a total amino acid concentration greater than about 50 mM. A further aspect of the invention relates to the use of fed batch and perfusion fermentation methods for cultivating polysaccharide-producing bacteria.

公开(公告)号：US20210180008A1

公开(公告)日：2021-06-17

申请号：US17170036

申请日：2021-02-08

Applicant: Pfizer Inc.

Inventor： Sunil Gururao Desai ,  Michael Allen Hanson ,  Jonathan Patrick Kinross ,  Daniel R. Lasko ,  Scott Ellis Lomberk ,  Jason Arnold Lotvin ,  Sujata Kaushikbhai Patel-Brown ,  Weiqiang Sun ,  Peter Anthony Tomasello

IPC: C12N1/20 ,  C12P19/04

Abstract: The present invention relates to media and fermentation methods for producing polysaccharides in bacterial cell culture. In one aspect, the invention relates to a complex culture medium comprising a vegetable hydrolysate, a yeast extract, and a carbon source. In another aspect, the invention relates to a defined media having a total amino acid concentration greater than about 50 mM. A further aspect of the invention relates to the use of fed batch and perfusion fermentation methods for cultivating polysaccharide-producing bacteria.

公开(公告)号：US20190292513A1

公开(公告)日：2019-09-26

申请号：US15775172

申请日：2016-11-10

Applicant: Pfizer Inc.

Inventor： Sunil Gururao Desai ,  Michael Allen Hanson ,  Jonathan Patrick Kinross ,  Daniel R. Lasko ,  Scott Ellis Lomberk ,  Jason Arnold Lotvin ,  Sujata Kaushikbhai Patel-Brown ,  Weiqiang Sun ,  Peter Anthony Tomasello

IPC: C12N1/20 ,  C12P19/04

Abstract: The present invention relates to media and fermentation methods for producing polysaccharides in bacterial cell culture. In one aspect, the invention relates to a complex culture medium comprising a vegetable hydrolysate, a yeast extract, and a carbon source. In another aspect, the invention relates to a defined media having a total amino acid concentration greater than about 50 mM. A further aspect of the invention relates to the use of fed batch and perfusion fermentation methods for cultivating polysaccharide-producing bacteria.