公开(公告)号：US20150204888A1

公开(公告)日：2015-07-23

申请号：US14537732

申请日：2014-11-10

申请人： Philip A. BAND ,  Hans-Georg WISNIEWSKI ,  Virginia BYERS KRAUS

发明人： Philip A. BAND ,  Hans-Georg WISNIEWSKI ,  Virginia BYERS KRAUS

IPC分类号： G01N33/68

CPC分类号： G01N33/6893 ,  G01N2333/4703 ,  G01N2333/811 ,  G01N2400/40 ,  G01N2800/105 ,  G01N2800/56 ,  G01N2800/7095

摘要： This invention relates to a method of predicting progression of an inflammatory condition in a subject, which involves providing a medium comprising hyaluronan or a fragment thereof; contacting the medium with a fluid sample from a subject with an inflammatory condition, where the fluid sample comprises proteins or proteoglycans and a transfer agent; incubating the fluid sample with the medium under conditions effective for the transfer agent in the fluid sample to mediate transfer of heavy chains from the proteins or proteoglycans to the hyaluronan or a fragment thereof to form a complex; detecting, using an antibody, occurrence levels of the complex; and comparing occurrence levels of the complex from said detecting to a reference standard to predict progression of an inflammatory condition in the subject. Also disclosed are methods of tailoring treatment of an inflammatory condition and quantifying local inflammatory activity in a body fluid.

摘要翻译： 本发明涉及一种预测受试者炎性病症进展的方法，其涉及提供包含透明质酸或其片段的培养基; 使来自受试者的流体样品与炎症状况接触，其中流体样品包含蛋白质或蛋白聚糖和转移剂; 在对流体样品中转移剂有效的条件下，将介质中的流体样品温育以介导重链从蛋白质或蛋白聚糖到透明质酸或其片段的转移以形成复合物; 使用抗体检测复合物的发生水平; 以及将来自所述检测的复合物的发生水平与参考标准进行比较，以预测受试者的炎症状态的进展。 还公开了定制炎性病症治疗和定量体液中的局部炎症活性的方法。

公开(公告)号：US20120028369A1

公开(公告)日：2012-02-02

申请号：US13172300

申请日：2011-06-29

申请人： Philip A. BAND ,  Hans-Georg WISNIEWSKI ,  Virginia Byers Kraus

发明人： Philip A. BAND ,  Hans-Georg WISNIEWSKI ,  Virginia Byers Kraus

IPC分类号： G01N33/53

CPC分类号： G01N33/6893 ,  G01N2333/4703 ,  G01N2333/811 ,  G01N2400/40 ,  G01N2800/105 ,  G01N2800/56 ,  G01N2800/7095

摘要： This invention relates to a method of predicting progression of an inflammatory condition in a subject, which involves providing a medium comprising hyaluronan or a fragment thereof; contacting the medium with a fluid sample from a subject with an inflammatory condition, where the fluid sample comprises proteins or proteoglycans and a transfer agent; incubating the fluid sample with the medium under conditions effective for the transfer agent in the fluid sample to mediate transfer of heavy chains from the proteins or proteoglycans to the hyaluronan or a fragment thereof to form a complex; detecting, using an antibody, occurrence levels of the complex; and comparing occurrence levels of the complex from said detecting to a reference standard to predict progression of an inflammatory condition in the subject. Also disclosed are methods of tailoring treatment of an inflammatory condition and quantifying local inflammatory activity in a body fluid.

摘要翻译： 本发明涉及一种预测受试者炎性病症进展的方法，其涉及提供包含透明质酸或其片段的培养基; 使来自受试者的流体样品与炎症状况接触，其中流体样品包含蛋白质或蛋白聚糖和转移剂; 在对流体样品中转移剂有效的条件下，将介质中的流体样品温育以介导重链从蛋白质或蛋白聚糖到透明质酸或其片段的转移以形成复合物; 使用抗体检测复合物的发生水平; 以及将来自所述检测的复合物的发生水平与参考标准进行比较，以预测受试者的炎症状态的进展。 还公开了定制炎性病症治疗和定量体液中的局部炎症活性的方法。

公开(公告)号：US20110206616A1

公开(公告)日：2011-08-25

申请号：US13013518

申请日：2011-01-25

申请人： Konstantin ICHTCHENKO ,  Philip A. BAND

发明人： Konstantin ICHTCHENKO ,  Philip A. BAND

IPC分类号： A61K38/16 ,  C07K14/33 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  C12N15/85 ,  C12N1/21 ,  C12N15/70 ,  C12P21/02 ,  A61K49/00 ,  C12Q1/02 ,  A61P19/02 ,  A61P25/00 ,  A61P25/06

CPC分类号： C12N9/52 ,  A61K38/00 ,  C07K14/33 ,  C07K2319/02 ,  C07K2319/036 ,  C07K2319/50 ,  G01N33/5058 ,  G01N2333/33 ,  Y02A50/469

摘要： This invention relates to isolated Clostridium botulinum propeptides and neurotoxins, isolated nucleic acid molecules encoding Clostridium botulinum propeptides and neurotoxins, methods of expression, treatment methods, and methods of detecting neurotoxin trafficking. The isolated Clostridium botulinum propeptides have a light chain region; a heavy chain region, where the light and heavy chain regions are linked by a disulfide bond; an intermediate region connecting the light and heavy chain regions and comprising a highly specific protease cleavage site; and an S6 peptide sequence according to SEQ ID NO:2 positioned upstream from, but not attached directly to, the N-terminus of the neurotoxin propeptide at the light chain region to enable site specific attachment of cargo.

摘要翻译： 本发明涉及分离的肉毒杆菌前肽和神经毒素，编码肉毒杆菌前列腺和神经毒素的分离的核酸分子，表达方法，治疗方法和检测神经毒素运输的方法。 分离的肉毒杆菌前肽具有轻链区域; 重链区，其中轻链和重链区通过二硫键连接; 连接轻链区和重链区并包含高度特异性蛋白酶切割位点的中间区; 以及位于轻链区域的位于神经毒素前肽的N-末端上游但不直接附着的SEQ ID NO：2的S6肽序列，以使得能够特异性地附着货物。