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公开(公告)号：US20110244477A1
公开(公告)日：2011-10-06
申请号：US13061085
申请日：2009-08-28
IPC分类号： G01N33/53 , G01N33/566
CPC分类号： G01N33/56972 , G01N33/6854 , G01N2333/16 , G01N2469/20 , G01N2800/24
摘要： An improved assay is described where a surface is provided with immobilized anti-Ig antibodies rather than antigen and where specific antibody-secreting cells (ASC) are detected using soluble antigen probes containing one of several possible labels. The method gives improved sensitivity with less background and is also more representative because antigen binding does not employ immobilized antigen. The assay is particularly effective for measuring antibody secreting cells against HIV, for determining whether an infection is acute as opposed to old or latent, for mapping epitopes and for measuring for ASCs against different antigens in the same reaction.
摘要翻译： 描述了一种改进的测定法，其中表面具有固定化抗Ig抗体而不是抗原，并且使用含有几种可能标记之一的可溶性抗原探针检测特异性抗体分泌细胞（ASC）。 该方法具有更少的背景的改善的灵敏度，并且也更具代表性，因为抗原结合不使用固定的抗原。 该测定对于测量针对HIV的抗体分泌细胞，用于确定感染是否与老的或潜伏期相比是急性的，用于测定表位并测量相同反应中针对不同抗原的ASC的测定。
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公开(公告)号：US10564160B2
公开(公告)日：2020-02-18
申请号：US13061085
申请日：2009-08-28
IPC分类号： G01N33/569
摘要： An improved assay is described where a surface is provided with immobilized anti-Ig antibodies rather than antigen and where specific antibody-secreting cells (ASC) are detected using soluble antigen probes containing one of several possible labels. The method gives improved sensitivity with less background and is also more representative because antigen binding does not employ immobilized antigen. The assay is particularly effective for measuring antibody secreting cells against HIV, for determining whether an infection is acute as opposed to old or latent, for mapping epitopes and for measuring for ASCs against different antigens in the same reaction.
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