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公开(公告)号：US20110244477A1
公开(公告)日：2011-10-06
申请号：US13061085
申请日：2009-08-28
IPC分类号： G01N33/53 , G01N33/566
CPC分类号： G01N33/56972 , G01N33/6854 , G01N2333/16 , G01N2469/20 , G01N2800/24
摘要： An improved assay is described where a surface is provided with immobilized anti-Ig antibodies rather than antigen and where specific antibody-secreting cells (ASC) are detected using soluble antigen probes containing one of several possible labels. The method gives improved sensitivity with less background and is also more representative because antigen binding does not employ immobilized antigen. The assay is particularly effective for measuring antibody secreting cells against HIV, for determining whether an infection is acute as opposed to old or latent, for mapping epitopes and for measuring for ASCs against different antigens in the same reaction.
摘要翻译： 描述了一种改进的测定法，其中表面具有固定化抗Ig抗体而不是抗原，并且使用含有几种可能标记之一的可溶性抗原探针检测特异性抗体分泌细胞（ASC）。 该方法具有更少的背景的改善的灵敏度，并且也更具代表性，因为抗原结合不使用固定的抗原。 该测定对于测量针对HIV的抗体分泌细胞，用于确定感染是否与老的或潜伏期相比是急性的，用于测定表位并测量相同反应中针对不同抗原的ASC的测定。
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公开(公告)号：US10564160B2
公开(公告)日：2020-02-18
申请号：US13061085
申请日：2009-08-28
IPC分类号： G01N33/569
摘要： An improved assay is described where a surface is provided with immobilized anti-Ig antibodies rather than antigen and where specific antibody-secreting cells (ASC) are detected using soluble antigen probes containing one of several possible labels. The method gives improved sensitivity with less background and is also more representative because antigen binding does not employ immobilized antigen. The assay is particularly effective for measuring antibody secreting cells against HIV, for determining whether an infection is acute as opposed to old or latent, for mapping epitopes and for measuring for ASCs against different antigens in the same reaction.
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公开(公告)号：US08790872B2
公开(公告)日：2014-07-29
申请号：US11587545
申请日：2005-04-29
CPC分类号： G01N33/54306 , G01N33/56983 , G01N33/6863
摘要： A method of detecting the production of an analyte by cells in a sample, which method comprises: (i) providing an antibody capable of binding to said analyte and a first agent capable of enhancing detection of said analyte, wherein said antibody and said first agent are immobilized on the same support; (ii) contacting said antibody and said agent with a sample of cells; and (iii) detecting binding of said analyte to said antibody thereby detecting the production of an analyte by cells in a sample.
摘要翻译： 一种检测样品中细胞产生分析物的方法，该方法包括：（i）提供能够结合所述分析物的抗体和能够增强所述分析物检测的第一试剂，其中所述抗体和所述第一试剂 固定在同一支架上; （ii）使所述抗体和所述药剂与细胞样品接触; 和（iii）检测所述分析物与所述抗体的结合，从而检测样品中细胞的分析物的产生。
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公开(公告)号：US08617808B2
公开(公告)日：2013-12-31
申请号：US11587350
申请日：2005-04-29
CPC分类号： G01N33/56972 , G01N33/54306 , G01N2500/00
摘要： The invention provides a method for detecting the production of one or more analytes by cells in a sample. The method uses one or more immobilized binding proteins to capture one or more analytes released from cells. The, or each, binding protein is localized at one or more discrete locations on a solid surface within a well. Cells are introduced into the well and cultured under conditions suitable for release of the analyte(s) and for binding of the analyte(s) to the binding protein(s). Restricting the binding protein(s) to one or more discrete locations within the well, rather than coating the entire surface of the well, results in increased sensitivity of the assay.
摘要翻译： 本发明提供一种用于检测样品中细胞产生一种或多种分析物的方法。 该方法使用一种或多种固定的结合蛋白来捕获从细胞释放的一种或多种分析物。 结合蛋白或每个结合蛋白被定位在孔内的固体表面上的一个或多个离散位置处。 将细胞引入孔中，并在适于释放分析物的条件下并在分析物与结合蛋白结合的条件下培养。 将结合蛋白限制在孔内的一个或多个离散位置，而不是涂覆孔的整个表面，导致测定的敏感性增加。
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公开(公告)号：US20080145837A1
公开(公告)日：2008-06-19
申请号：US11587545
申请日：2005-04-29
CPC分类号： G01N33/54306 , G01N33/56983 , G01N33/6863
摘要： A method of detecting the production of an analyte by cells in a sample, which method comprises: (i) providing an antibody capable of binding to said analyte and a first agent capable of enhancing detection of said analyte, wherein said antibody and said first agent are immobilised on the same support; (ii) contacting said antibody and said agent with a sample of cells; and (iii) detecting binding of said analyte to said antibody thereby detecting the production of an analyte by cells in a sample.
摘要翻译： 一种检测样品中细胞产生分析物的方法，该方法包括：（i）提供能够结合所述分析物的抗体和能够增强所述分析物检测的第一试剂，其中所述抗体和所述第一试剂 固定在同一支架上; （ii）使所述抗体和所述药剂与细胞样品接触; 和（iii）检测所述分析物与所述抗体的结合，从而检测样品中细胞的分析物的产生。
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公开(公告)号：US20070178449A1
公开(公告)日：2007-08-02
申请号：US11587350
申请日：2005-04-29
IPC分类号： C12Q1/70 , G01N33/567 , G01N33/574
CPC分类号： G01N33/56972 , G01N33/54306 , G01N2500/00
摘要： The invention provides a method for detecting the production by cells in a sample of one or more analyte, which method comprises: (a) providing (i) a first binding protein which specifically binds to a first analyte; and (ii) optionally a second binding protein which specifically binds to second analyte; wherein said first binding protein and optionally said second binding protein are each immobilized at one or more discrete location within a well; (b) introducing a sample of cells into said well; (c) culturing said sample of cells under conditions suitable for: (i) release of said analyte and optionally said second analyte and optionally said one or more further analyte by said cells; and (ii) binding of said first analyte to said first binding protein and optionally binding of said second analyte to said second binding protein; and (d) determining whether or not said first analyte is bound to said first binding protein and whether or not said second analyte is bound to said second binding protein, thereby detecting production by a sample of cells of one or more analyte.
摘要翻译： 本发明提供了一种用于检测一种或多种分析物的样品中细胞产生的方法，该方法包括：（a）提供（i）特异性结合第一分析物的第一结合蛋白; 和（ii）任选地特异性结合第二分析物的第二结合蛋白; 其中所述第一结合蛋白和任选地所述第二结合蛋白各自固定在孔内的一个或多个离散位置处; （b）将细胞样品引入所述孔中; （c）在适于以下条件下培养所述细胞样品：（i）所述细胞释放所述分析物和任选地所述第二分析物和任选所述一种或多种另外的分析物; 和（ii）所述第一分析物与所述第一结合蛋白的结合以及所述第二分析物与所述第二结合蛋白的任选结合; 和（d）确定所述第一分析物是否与所述第一结合蛋白结合，以及所述第二分析物是否与所述第二结合蛋白结合，从而检测一种或多种分析物的细胞样品的产生。
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