公开(公告)号：US20140189907A1

公开(公告)日：2014-07-03

申请号：US13729543

申请日：2012-12-28

申请人： Ping Song ,  Laura Ann Tagliani ,  John William Pellow

发明人： Ping Song ,  Laura Ann Tagliani ,  John William Pellow

IPC分类号： C12N15/82

CPC分类号： C12N15/8286 ,  C12N15/8213 ,  Y02A40/162

摘要： This invention relates to plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell.

摘要翻译： 本发明涉及植物育种和植物对昆虫的保护。 更具体地，本发明包括棉花植物的新转化事件，其包含插入到棉花细胞基因组内的特定位点的如本文所述的一个或多个多核苷酸序列。

公开(公告)号：US07883850B2

公开(公告)日：2011-02-08

申请号：US11704418

申请日：2007-02-09

申请人： Ping Song ,  Laura Ann Tagliani ,  John William Pellow

发明人： Ping Song ,  Laura Ann Tagliani ,  John William Pellow

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12N15/8286 ,  C07K14/325 ,  C12Q1/6895 ,  Y02A40/162

摘要： This invention relates to plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell. In highly preferred embodiments, said polynucleotide sequences encode “stacked” Cry1F and Cry1Ac lepidopteran insect inhibitory proteins. However, the subject invention includes plants having single cry1F or cry1Ac events, as described herein. Additionally, the invention is related to cotton plants derived from that transformation event and to assays for detecting the presence of the event in a sample. More specifically, the present invention provides DNA and related assays for detecting the presence of certain insect-resistance events in cotton. The assays are based on the DNA sequences of recombinant constructs inserted into the cotton genome and of the genomic sequences flanking the insertion sites. These sequences are unique. Based on these insert and border sequences, event-specific primers were generated. PCR analysis demonstrated that these cotton lines can be identified in different cotton genotypes by analysis of the PCR amplicons generated with these event-specific primer sets. Thus, these and other related procedures can be used to uniquely identify these cotton lines. Kits and conditions useful in conducting the assays are also provided. These materials and methods can also be used to assist breeding programs to further develop traits in cotton.

摘要翻译： 本发明涉及植物育种和植物对昆虫的保护。 更具体地，本发明包括棉花植物的新转化事件，其包含插入到棉花细胞基因组内的特定位点的如本文所述的一个或多个多核苷酸序列。 在非常优选的实施方案中，所述多核苷酸序列编码“堆积”Cry1F和Cry1Ac鳞翅目昆虫抑制蛋白。 然而，本发明包括如本文所述的具有单一cry1F或cry1Ac事件的植物。 此外，本发明涉及来自该转化事件的棉花植物和用于检测样品中事件的存在的测定法。 更具体地，本发明提供用于检测棉花中某些昆虫抗性事件的存在的DNA和相关测定法。 测定法基于插入棉花基因组的重组构建体的DNA序列和插入位点侧翼的基因组序列。 这些序列是唯一的。 基于这些插入和边界序列，生成事件特异性引物。 PCR分析表明，通过分析用这些事件特异性引物组产生的PCR扩增子，可以在不同棉花基因型中鉴定这些棉花品系。 因此，这些和其他相关程序可用于唯一地识别这些棉花线。 还提供了用于进行测定的试剂盒和条件。 这些材料和方法也可用于协助育种计划进一步发展棉花性状。

公开(公告)号：US07179965B2

公开(公告)日：2007-02-20

申请号：US10964838

申请日：2004-10-13

申请人： Ping Song ,  Laura Ann Tagliani ,  John William Pellow

发明人： Ping Song ,  Laura Ann Tagliani ,  John William Pellow

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82

CPC分类号： C12N15/8286 ,  C07K14/325 ,  C12Q1/6895 ,  Y02A40/162

摘要： This invention relates to plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell. In highly preferred embodiments, said polynucleotide sequences encode “stacked” Cry1F and Cry1Ac lepidopteran insect inhibitory proteins. However, the subject invention includes plants having single cry1F or cry1Ac events, as described herein. Additionally, the invention is related to cotton plants derived from that transformation event and to assays for detecting the presence of the event in a sample. More specifically, the present invention provides DNA and related assays for detecting the presence of certain insect-resistance events in cotton. The assays are based on the DNA sequences of recombinant constructs inserted into the cotton genome and of the genomic sequences flanking the insertion sites. These sequences are unique. Based on these insert and border sequences, event-specific primers were generated. PCR analysis demonstrated that these cotton lines can be identified in different cotton genotypes by analysis of the PCR amplicons generated with these event-specific primer sets. Thus, these and other related procedures can be used to uniquely identify these cotton lines. Kits and conditions useful in conducting the assays are also provided. These materials and methods can also be used to assist breeding programs to further develop traits in cotton.

摘要翻译： 本发明涉及植物育种和植物对昆虫的保护。 更具体地，本发明包括棉花植物的新转化事件，其包含插入到棉花细胞基因组内的特定位点的如本文所述的一个或多个多核苷酸序列。 在非常优选的实施方案中，所述多核苷酸序列编码“堆积”Cry1F和Cry1Ac鳞翅目昆虫抑制蛋白。 然而，本发明包括如本文所述的具有单一cry1F或cry1Ac事件的植物。 此外，本发明涉及来自该转化事件的棉花植物和用于检测样品中事件的存在的测定法。 更具体地，本发明提供用于检测棉花中某些昆虫抗性事件的存在的DNA和相关测定法。 测定法基于插入棉花基因组的重组构建体的DNA序列和插入位点侧翼的基因组序列。 这些序列是唯一的。 基于这些插入和边界序列，生成事件特异性引物。 PCR分析表明，通过分析用这些事件特异性引物组产生的PCR扩增子，可以在不同棉花基因型中鉴定这些棉花品系。 因此，这些和其他相关程序可用于唯一地识别这些棉花线。 还提供了用于进行测定的试剂盒和条件。 这些材料和方法也可用于协助育种计划进一步发展棉花性状。

公开(公告)号：US20110191900A1

公开(公告)日：2011-08-04

申请号：US13021410

申请日：2011-02-04

申请人： Ping Song ,  Laura Ann Tagllani ,  John William Pellow

发明人： Ping Song ,  Laura Ann Tagllani ,  John William Pellow

IPC分类号： A01H1/00 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8286 ,  C07K14/325 ,  C12Q1/6895 ,  Y02A40/162

摘要： This invention relates to plant breeding and the protection of plants from insects. More specifically, this invention includes novel transformation events of cotton plants comprising one or more polynucleotide sequences, as described herein, inserted into specific site(s) within the genome of a cotton cell. In highly preferred embodiments, said polynucleotide sequences encode “stacked” Cry1F and Cry1Ac lepidopteran insect inhibitory proteins. However, the subject invention includes plants having single cry1F or cry1Ac events, as described herein. Additionally, the invention is related to cotton plants derived from that transformation event and to assays for detecting the presence of the event in a sample. More specifically, the present invention provides DNA and related assays for detecting the presence of certain insect-resistance events in cotton. The assays are based on the DNA sequences of recombinant constructs inserted into the cotton genome and of the genomic sequences flanking the insertion sites. These sequences are unique. Based on these insert and border sequences, event-specific primers were generated. PCR analysis demonstrated that these cotton lines can be identified in different cotton genotypes by analysis of the PCR amplicons generated with these event-specific primer sets. Thus, these and other related procedures can be used to uniquely identify these cotton lines. Kits and conditions useful in conducting the assays are also provided. These materials and methods can also be used to assist breeding programs to further develop traits in cotton.

摘要翻译： 本发明涉及植物育种和植物对昆虫的保护。 更具体地，本发明包括棉花植物的新转化事件，其包含插入到棉花细胞基因组内的特定位点的如本文所述的一个或多个多核苷酸序列。 在非常优选的实施方案中，所述多核苷酸序列编码“堆积”Cry1F和Cry1Ac鳞翅目昆虫抑制蛋白。 然而，本发明包括如本文所述的具有单一cry1F或cry1Ac事件的植物。 此外，本发明涉及来自该转化事件的棉花植物和用于检测样品中事件的存在的测定法。 更具体地，本发明提供用于检测棉花中某些昆虫抗性事件的存在的DNA和相关测定法。 测定法基于插入棉花基因组的重组构建体的DNA序列和插入位点侧翼的基因组序列。 这些序列是唯一的。 基于这些插入和边界序列，生成事件特异性引物。 PCR分析表明，通过分析用这些事件特异性引物组产生的PCR扩增子，可以在不同棉花基因型中鉴定这些棉花品系。 因此，这些和其他相关程序可用于唯一地识别这些棉花线。 还提供了用于进行测定的试剂盒和条件。 这些材料和方法也可用于协助育种计划进一步发展棉花性状。

公开(公告)号：US07332657B2

公开(公告)日：2008-02-19

申请号：US11013961

申请日：2004-12-16

申请人： Joel Frank Mahill ,  John William Pellow

发明人： Joel Frank Mahill ,  John William Pellow

IPC分类号： A01H5/00 ,  A01H5/10 ,  A01H4/00 ,  A01H1/00 ,  C12N15/82

CPC分类号： A01H5/10

摘要： A cotton cultivar, designated PHY 800 Pima, is disclosed. The invention relates to the seeds of cotton cultivar PHY 800 Pima, to the plants and plant parts of cotton cultivar PHY 800 Pima and to methods for producing a cotton plant produced by crossing the cultivar PHY 800 Pima with itself or another cotton variety. The invention further relates to hybrid cotton seeds and plants produced by crossing the cultivar PHY 800 Pima with another cotton cultivar.

摘要翻译： 披露了一种称为PHY 800 Pima的棉花品种。 本发明涉及棉花品种PHY 800 Pima的种子，棉花品种PHY 800 Pima的植物和植物部分以及通过将品种PHY 800 Pima与其本身或另一种棉花品种杂交而生产的棉花植物的方法。 本发明还涉及通过将品种PHY 800 Pima与另一棉花品种杂交而产生的杂种棉花种子和植物。

公开(公告)号：US20080295195A1

公开(公告)日：2008-11-27

申请号：US12012370

申请日：2008-02-01

申请人： Joel Frank Mahill ,  John William Pellow

发明人： Joel Frank Mahill ,  John William Pellow

IPC分类号： A01H5/00 ,  A01H1/02

CPC分类号： A01H5/10

摘要： A cotton cultivar, designated PHY 800 Pima, is disclosed. The invention relates to the seeds of cotton cultivar PHY 800 Pima, to the plants and plant parts of cotton cultivar PHY 800 Pima and to methods for producing a cotton plant produced by crossing the cultivar PHY 800 Pima with itself or another cotton variety. The invention further relates to hybrid cotton seeds and plants produced by crossing the cultivar PHY 800 Pima with another cotton cultivar.

公开(公告)号：US07038116B2

公开(公告)日：2006-05-02

申请号：US11013960

申请日：2004-12-16

申请人： Joel Frank Mahill ,  John William Pellow

发明人： Joel Frank Mahill ,  John William Pellow

IPC分类号： A01H5/00 ,  A01H5/10 ,  A01H1/00 ,  A01H4/00 ,  C12N15/82

CPC分类号： A01H5/10

摘要： A cotton cultivar designated PHY 78 Acala is disclosed. The invention relates to the seeds of cotton cultivar PHY 78 Acala, to the plants and plant parts of cotton PHY 78 Acala and to methods for producing a cotton plant produced by crossing the cultivar PHY 78 Acala with itself or another cotton variety. The invention further relates to hybrid cotton seeds and plants produced by crossing the cultivar PHY 78 Acala with another cotton cultivar.

摘要翻译： 披露了一种名为PHY 78 Acala的棉花品种。 本发明涉及棉花品种PHY 78 Acala的种子，棉花PHY 78 Acala的植物和植物部分以及通过将品种PHY 78 Acala与其本身或另一种棉花品种杂交产生的棉花植物的方法。 本发明还涉及通过将品种PHY 78 Acala与另一种棉花栽培品种杂交而产生的杂种棉花种子和植物。