公开(公告)号：US08158402B2

公开(公告)日：2012-04-17

申请号：US11667567

申请日：2005-11-04

申请人： Ping Xu ,  Zijun Xiao ,  Yi Du ,  Zhonghao Wei

发明人： Ping Xu ,  Zijun Xiao ,  Yi Du ,  Zhonghao Wei

IPC分类号： C12N1/20 ,  C12P7/26 ,  C12P1/00

CPC分类号： C12P7/26 ,  C12N1/20 ,  C12R1/07

摘要： A high-yield bacterial strain for producing acetoin named Bacillus pumilus XH195 has been deposited in a microorganism deposit center in Germany. The deposit number is DSM 16187. The bacteria of the strain are rod-shaped, 1.5 μm to 3.0 μm in length, and 0.6 μm to 0.7 μm in diameter. The colony color of the bacterial strain is yellow or white. The strain has the typical fatty acid profile of Bacillus and its physiological and biochemical characteristics align with those of Bacillus pumilus. When the bacteria of strain were cultured at 37° C. for 60 hours with shaking, the yield of acetoin could reach 63.0 g/L or 58.1 g/L, when glucose or sucrose was used as the substrate, respectively.

摘要翻译： 用于生产称为短小芽孢杆菌XH195的丙酮丁酸的高产菌株已经存放在德国的微生物保藏中心。 保藏号为DSM16187.菌株细菌为棒状，长度为1.5〜3.0μm，直径为0.6〜0.7μm。 菌株的菌落颜色是黄色或白色。 该菌株具有芽孢杆菌的典型脂肪酸谱，其生理和生化特性与短小芽孢杆菌的一致。 当菌株细菌在37℃下振荡培养60小时时，分别使用葡萄糖或蔗糖作为底物时，乙偶姻的产量可达63.0g / L或58.1g / L。

公开(公告)号：US08067212B2

公开(公告)日：2011-11-29

申请号：US12302275

申请日：2007-05-25

申请人： Ping Xu ,  Zijun Xiao ,  Zhonghao Wei ,  Yi Du

发明人： Ping Xu ,  Zijun Xiao ,  Zhonghao Wei ,  Yi Du

IPC分类号： C12P17/00 ,  C12N1/20

CPC分类号： C12R1/07 ,  C12P17/12 ,  Y10S435/832

摘要： This invention provides a high-yield bacterial strain for producing tetramethylpyrazine named Bacillus pumilus RX3-17. The strain has been deposited in China Center for Type Culture Collection on Apr. 19, 2006. The deposit number is CCTCC M 206043. The bacterial strain, isolated from soil, is rod-shaped, 1.5 μm to 3.0 μm in length and 0.6 μm to 0.7 μm in diameter. The colony color of the strain is milky-white. The strain has typical physiological and biochemical characteristics of Bacillus pumilus. The 16s rDNA sequence of this strain shares a similarity of 99% with other Bacillus pumilus strains. This invention belongs to the domain of biotechnology. The strain can be applied to the production of tetramethylpyrazine with glucose as the substrate, solving the bottleneck of low yield in bacterial tetramethylpyrazine fermentation.

摘要翻译： 本发明提供了用于生产称为短小芽孢杆菌RX3-17的四甲基吡嗪的高产菌株。 该菌株已于2006年4月19日保藏在中国型号培养物中心中心。沉积物编号为CCTCC M 206043.从土壤中分离的细菌菌株为棒状，长度为1.5〜3.0μm，0.6μm 直径为0.7μm。 菌株的菌落颜色是乳白色的。 该菌株具有典型的生物和生化特性的短小芽孢杆菌。 该菌株的16s rDNA序列与其他短小芽孢杆菌菌株具有99％的相似性。 本发明属于生物科技领域。 该菌株可用于以葡萄糖为底物生产四甲基吡嗪，解决细菌四甲基吡嗪发酵中低产量的瓶颈。

公开(公告)号：US20090275108A1

公开(公告)日：2009-11-05

申请号：US12302275

申请日：2007-05-25

申请人： Ping Xu ,  Zijun Xiao ,  Zhonghao Wei ,  Yi Du

发明人： Ping Xu ,  Zijun Xiao ,  Zhonghao Wei ,  Yi Du

IPC分类号： C12N1/20

CPC分类号： C12R1/07 ,  C12P17/12 ,  Y10S435/832

摘要： This invention provides a high-yield bacterial strain for producing tetramethylpyrazine named Bacillus pumilus RX3-17. The strain has been deposited in China Center for Type Culture Collection on Apr. 19, 2006. The deposit number is CCTCC M 206043. The bacterial strain, isolated from soil, is rod-shaped, 1.5 μm to 3.0 μm in length and 0.6 μm to 0.7 μm in diameter. The colony color of the strain is milky-white. The strain has typical physiological and biochemical characteristics of Bacillus pumilus. The 16s rDNA sequence of this strain shares a similarity of 99% with other Bacillus pumilus strains. This invention belongs to the domain of biotechnology. The strain can be applied to the production of tetramethylpyrazine with glucose as the substrate, solving the bottleneck of low yield in bacterial tetramethylpyrazine fermentation.

摘要翻译： 本发明提供了用于生产称为短小芽孢杆菌RX3-17的四甲基吡嗪的高产菌株。 该菌株于2006年4月19日存放于中国型号培养物保藏中心。存款编号为CCTCC M 206043.从土壤中分离出的菌株为棒状，长度为1.5〜3.0μm，长度为0.6μm 直径为0.7毫米。 菌株的菌落颜色是乳白色的。 该菌株具有典型的生物和生化特性的短小芽孢杆菌。 该菌株的16s rDNA序列与其他短小芽孢杆菌菌株具有99％的相似性。 本发明属于生物技术领域。 该菌株可用于以葡萄糖为底物生产四甲基吡嗪，解决细菌四甲基吡嗪发酵中低产量的瓶颈。

公开(公告)号：US20090215152A1

公开(公告)日：2009-08-27

申请号：US11667567

申请日：2005-11-04

申请人： Ping Xu ,  Zijun Xiao ,  Yi Du ,  Zhonghao Wei

发明人： Ping Xu ,  Zijun Xiao ,  Yi Du ,  Zhonghao Wei

IPC分类号： C12N1/20

CPC分类号： C12P7/26 ,  C12N1/20 ,  C12R1/07

摘要： A high-yield bacterial strain for producing acetoin named Bacillus pumilus XH195 has been deposited in a microorganism deposit center in Germany. The deposit number is DSM 16187. The bacteria of the strain are rod-shaped, 1.5 μm to 3.0 μm in length, and 0.6 μm to 0.7 μm in diameter. The colony color of the bacterial strain is yellow or white. The strain has the typical fatty acid profile of Bacillus and its physiological and biochemical characteristics align with those of Bacillus pumilus. When the bacteria of strain were cultured at 37° C. for 60 hours with shaking, the yield of acetoin could reach 63.0 g/L or 58.1 g/L, when glucose or sucrose was used as the substrate, respectively.

摘要翻译： 用于生产称为短小芽孢杆菌XH195的丙酮丁酸的高产菌株已经存放在德国的微生物保藏中心。 存款编号为DSM16187.菌株的细菌为棒状，长度为1.5μm至3.0μm，直径为0.6μm至0.7μm。 菌株的菌落颜色是黄色或白色。 该菌株具有芽孢杆菌的典型脂肪酸谱，其生理和生化特性与短小芽孢杆菌的一致。 当菌株细菌在37℃下振荡培养60小时时，分别使用葡萄糖或蔗糖作为底物时，乙偶姻的产量可达63.0g / L或58.1g / L。

公开(公告)号：US20090186399A1

公开(公告)日：2009-07-23

申请号：US12302502

申请日：2007-06-25

申请人： Ping Xu ,  Dongliang Hua ,  Shan Lin ,  Lifu Song ,  Cuiqing Ma ,  Zhaobin Zhang ,  Yi Du ,  Hong Chen ,  Lin Gan ,  Zhonghao Wei ,  Yiyong Zeng

发明人： Ping Xu ,  Dongliang Hua ,  Shan Lin ,  Lifu Song ,  Cuiqing Ma ,  Zhaobin Zhang ,  Yi Du ,  Hong Chen ,  Lin Gan ,  Zhonghao Wei ,  Yiyong Zeng

IPC分类号： C12N1/20

CPC分类号： C12R1/465 ,  C12P7/24

摘要： A method of vanillin production from ferulic acid with a high concentration by biotransformation using a Streptomyces sp. strain is claimed in this invention. This strain is named as Streptomyces sp. V-1, which has been deposited in China Center for Type Culture Collection on Jul. 12, 2006 with the number of CCTCC M 206065. Using this strain, high concentration of vanillin fermentation broth is obtained from ferulic acid by biotransformation in GY biotransformation medium. With the addition of macroporous adsorbent resin DM11, the concentration of vanillin can be greatly improved. The advantage of this invention is less environmental pollution, high product concentration, less by-product, short processing cycle, low production cost, simple product extraction, clean production process, product environmental friendly, safe and reliable, which solves many difficulties in the vanillin production from botanical raw material extraction or chemical synthesis, and therefore it has good application prospect.

摘要翻译： 通过使用链霉菌（Streptomyces sp。）通过生物转化从高浓度的阿魏酸生产香草醛的方法。 应变在本发明中要求保护。 该菌株被命名为Streptomyces sp。 V-1已于2006年7月12日以CCTCC M 206065号存于中国型号培养物中心中心。使用该菌株，通过生物转化在GY生物转化培养基中从阿魏酸获得高浓度的香草醛发酵液 。 通过添加大孔吸附树脂DM11，可以大大提高香草醛的浓度。 本发明的优点是环境污染少，产品浓缩少，加工周期短，生产成本低，产品提取简单，生产过程简单，产品环保，安全可靠，解决了香草醛中的许多困难 生产从植物原料提取或化学合成，因此具有良好的应用前景。