公开(公告)号：US20130313112A1

公开(公告)日：2013-11-28

申请号：US13962141

申请日：2013-08-08

Applicant: President and Fellows of Harvard College

Inventor： Timothy J. DENISON ,  Alexis Sauer-Budge ,  Jene A. Golovchenko ,  Amit Meller ,  Eric Brandin ,  Daniel Branton

IPC: G01N27/447

CPC classification number: C12Q1/6869 ,  G01N27/447 ,  G01N33/48721 ,  C12Q2565/631 ,  C12Q2565/607

Abstract: The invention relates to a method for characterizing a target polynucleic acid by providing a surface containing a channel of a dimension sufficient to allow sequential monomer-by-monomer passage of a single-stranded polynucleic acid, but not of a double-stranded polynucleic acid; providing a source of hybridized target polynucleic acid at the surface; inducing passage of the target polynucleic acid through the channel, whereby the target polynucleic acid undergoes base pair separation (melts) prior to its passage; and making one or more measurements over time as the target polynucleic acid moves relative to the channel yielding data suitable to determine a monomer-dependent characteristic of the target polynucleic acid.

公开(公告)号：US20130270115A1

公开(公告)日：2013-10-17

申请号：US13852606

申请日：2013-03-28

Applicant: President and Fellows of Harvard College

Inventor： Timothy J. DENISON ,  Alexis Sauer-Budge ,  Jene A. Golovchenko ,  Amit Meller ,  Eric Brandin ,  Daniel Branton

IPC: G01N27/447

CPC classification number: C12Q1/6869 ,  G01N27/447 ,  G01N33/48721 ,  C12Q2565/631 ,  C12Q2565/607

Abstract: The invention relates to a method for characterizing a target polynucleic acid by providing a surface containing a channel of a dimension sufficient to allow sequential monomer-by-monomer passage of a single-stranded polynucleic acid, but not of a double-stranded polynucleic acid; providing a source of hybridized target polynucleic acid at the surface; inducing passage of the target polynucleic acid through the channel, whereby the target polynucleic acid undergoes base pair separation (melts) prior to its passage; and making one or more measurements over time as the target polynucleic acid moves relative to the channel yielding data suitable to determine a monomer-dependent characteristic of the target polynucleic acid.

Abstract translation: 本发明涉及通过提供含有足以允许单链单体通过单链多核酸而不是双链多核酸的尺寸的通道的表面来表征目标多核酸的方法; 在表面提供杂交靶标多核酸的来源; 诱导目标多核酸通过通道，由此目标多核酸在其通过之前经历碱基对分离（熔融）; 并且随着靶多核酸相对于通道产生适合于确定目标多核苷酸的单体依赖特性的数据，随时间进行一个或多个测量。