公开(公告)号：US20240002814A1

公开(公告)日：2024-01-04

申请号：US18035149

申请日：2021-11-03

Applicant: Protalix Ltd.

Inventor： Ilya RUDERFER ,  Yakir NATAF ,  Gil ARVATZ ,  Uri HANANIA ,  Tamar ARIEL ,  Shelly ROZEN ,  Yael HAYON

IPC: C12N9/06 ,  A61K38/44

CPC classification number: C12N9/0048 ,  A61K38/44 ,  C12Y107/03003

Abstract: A modified uricase is described herein, as well as a method of reducing a level of uric acid by contacting a medium with the modified uricase. The modified uricase comprises a uricase polypeptide crosslinked by at least one bifunctional linking moiety that comprises a poly(alkylene glycol) moiety. A molecular weight of the bifunctional linking moiety is from about 1.5 kDa to about 4 kDa, and/or the modified uricase comprises a plurality of polypeptides having the amino acid sequence SEQ ID NO: 2. Further described is a polypeptide having the amino acid sequence SEQ ID NO: 2. A process of preparing the modified uricase is also described, comprising contacting the polypeptide with a crosslinking agent that comprises a poly(alkylene glycol) moiety and at least two aldehyde groups, to obtain a conjugate; and contacting the conjugate with a reducing agent.

公开(公告)号：US20240263150A1

公开(公告)日：2024-08-08

申请号：US18429649

申请日：2024-02-01

Applicant: Protalix Ltd.

Inventor： Ilya RUDERFER ,  Yakir NATAF ,  Gil ARVATZ ,  Uri HANANIA ,  Tamar ARIEL ,  Shelly ROZEN ,  Yael HAYON ,  Zohar KEREN ,  Orit COHEN-BARAK

IPC: C12N9/06 ,  A61K9/00 ,  A61K31/519

CPC classification number: C12N9/0048 ,  A61K9/0019 ,  A61K31/519

Abstract: A method of treating a disease or disorder associated with excessive uric acid levels is disclosed herein. The method comprises administering to the subject by i.v. infusion an amount of a recombinant homotetrameric uricase enzyme comprising four uricase polypeptides having the amino acid sequence as set forth in SEQ ID NO: 2, wherein the polypeptides are crosslinked by polyethylene glycol (PEG) bis-aldehyde having a molecular weight of 2-3.5 kDa.