公开(公告)号：US20230022576A1

公开(公告)日：2023-01-26

申请号：US17777669

申请日：2019-11-19

Applicant: Protalix Ltd.

Inventor： Maor SHEVA ,  Uri HANANIA

IPC: C12N15/82 ,  C12N15/11 ,  C12N9/22

Abstract: A nucleic acid construct is disclosed which is removable after transformation. Methods of using same are disclosed as well.

公开(公告)号：US20230374447A1

公开(公告)日：2023-11-23

申请号：US18030103

申请日：2021-10-05

Applicant: Protalix Ltd.

Inventor： Uri HANANIA ,  Maor SHEVA

IPC: C12N5/04 ,  C12N15/82 ,  C12N9/22

CPC classification number: C12N5/04 ,  C12N15/8257 ,  C12N9/22 ,  C12N2310/20

Abstract: DICER-liker knock-out plant cells are provided. Accordingly, there is provided an isolated plant cell in suspension comprising loss of function mutations in all alleles of at least two genes selected from the group consisting of DCL2, DCL4, RDR1, RDR2 and RDR6 in said plant cell. Also provided are methods of abolishing expression and/or activity of at least two genes selected from the group consisting of DCL2, DCL4, RDR1, RDR2 and RDR6 in a plant cell.