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公开(公告)号：US20210054048A1
公开(公告)日：2021-02-25
申请号：US16975431
申请日：2019-02-25
Applicant: Purdue Research Foundation
Inventor： David Harley Thompson , Craig Sweet , Aayush Aayush , Ross VerHeul
Abstract: Disclosed herein is an optimized Elastin-like peptide purification scheme from whole cell lysate that is broadly applicable to neutral, acidic or basic ELP polypeptide. The method involves the use of one or more organic solvent to extract the ELP polypeptide from whole lysate followed by optional back extraction, precipitation, hot spins, and/or dialysis to purify the target ELP polypeptide.
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