公开(公告)号：US20230192601A1

公开(公告)日：2023-06-22

申请号：US17924865

申请日：2021-03-09

Applicant: Purdue Research Foundation

Inventor： David Harley Thompson ,  Zoltan Kalman Nagy ,  Ahmed Mufti ,  Jaron Mackey

IPC: C07C273/18

CPC classification number: C07C273/18

Abstract: The present disclosure provides processes and apparatuses for the scaled-up manufacture of lomustine via continuous flow manufacture. Such continuous flow processes may optionally include the crystallization of lomustine and the apparatuses may optionally include crystallization apparatuses/reactors in either batch or continuous flow design. In one aspect of the disclosure, a process for making lomustine is provided comprising treating solutions of 2-chloroethylisocyanate with a solution of cyclohexylamine with continuous-flow pumps in a gram-flow reactor to form a combined solution, adding deionized water with a continuous flow-pump to the combined solution to form a liquid-organic phase solution, extracting the organic phase from the solution and treating with a solution of t-butyl nitrite with a continuous flow pump in a gram flow reactor to form lomustine.

公开(公告)号：US20230183246A1

公开(公告)日：2023-06-15

申请号：US17926379

申请日：2021-05-13

Applicant: Purdue Research Foundation

Inventor： David Harley Thompson ,  Herman O. Sintim ,  Qingqing Qi ,  Shruti Biyani

IPC: C07D487/04 ,  C07D401/06 ,  C07D471/04 ,  B01J31/24

CPC classification number: C07D487/04 ,  C07D401/06 ,  C07D471/04 ,  B01J31/2404 ,  B01J2531/824 ,  B01J2231/32

Abstract: The present disclosure relates to a telescoped continuous flow Sonogashira coupling synthesis for some lead compounds to support in vivo studies and pre-clinical evaluation. The application of high throughput tools combined with the telescoped continuous synthesis method can enable an efficient and safe synthesis of compounds of interest involving hazardous coupling reagents such as HATU, while minimizing by-product formation.

公开(公告)号：US20210054048A1

公开(公告)日：2021-02-25

申请号：US16975431

申请日：2019-02-25

Applicant: Purdue Research Foundation

Inventor： David Harley Thompson ,  Craig Sweet ,  Aayush Aayush ,  Ross VerHeul

IPC: C07K14/78 ,  C07K1/13

Abstract: Disclosed herein is an optimized Elastin-like peptide purification scheme from whole cell lysate that is broadly applicable to neutral, acidic or basic ELP polypeptide. The method involves the use of one or more organic solvent to extract the ELP polypeptide from whole lysate followed by optional back extraction, precipitation, hot spins, and/or dialysis to purify the target ELP polypeptide.