公开(公告)号：US20180275118A1

公开(公告)日：2018-09-27

申请号：US15762597

申请日：2016-09-24

Applicant: Purdue Research Foundation

Inventor： Baird Zane ,  Robert Graham Cooks ,  Adam Hollerbach ,  Zheng Ouyang ,  Michael Pugia

IPC: G01N33/53

CPC classification number: G01N33/5302 ,  G01N2458/15

Abstract: The invention generally relates to mass tag analysis for rare cells and cell free molecules. In certain embodiments, the invention provides an apparatus including an essentially non-absorbent membrane having at least one pore, a microwell operably associated with the essentially non-absorbent membrane, and an electric field generator. The apparatus may be configured such that an electric field produced by the electric field generator operably interacts with a sample in the microwell and expels a droplet of the sample through the at least one pore in the essentially non-absorbent membrane. In certain embodiments, apparatuses of the invention are used for detection, and optionally quantification, of a target analyte from a heterogeneous sample, such as a rare target analyte (e.g., rare cell) from a biological sample.