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公开(公告)号：US20200010906A1
公开(公告)日：2020-01-09
申请号：US16494949
申请日：2018-03-01
申请人： Qiagen GmbH
IPC分类号： C12Q1/6886 , G16B40/10 , G16H50/30
摘要： Provided is a method for analysing the expression of one or more biomarker RNA molecules, comprising (A) isolating RNA from circulating tumor cells obtained from a subject, determining the expression of at least one biomarker RNA molecule in the isolated RNA and providing an expression profile based on the results; (B) isolating RNA from extracellular vesicles obtained from the subject, determining the expression of at least one biomarker RNA molecule in the isolated RNA and providing an expression profile based on the results; and (C)using the expression profiles determined in (A) and determined in (B) for a combined analysis of the results. Such combined analysis of the CTC and EV expression profiles enhances the prognostic and predictive value of the obtained results and can provide valuable diagnostic, prognostic and/or predictive information. The present method can thus be used as improved diagnostic, prognostic and/or predictive aid in the management of cancer patients. It can be used to support the diagnosis, prognosis or to choose the most appropriate treatment for cancer patients.
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公开(公告)号：US20220259637A1
公开(公告)日：2022-08-18
申请号：US17620612
申请日：2020-06-26
申请人： QIAGEN GmbH
IPC分类号： C12Q1/6806 , C12Q1/686 , C12N15/10 , C12N15/82
摘要： The present invention provides a poly(alkylene oxide) polymer based size selective method for enriching nucleic acid molecules having a length below a cut-off value from a nucleic acid containing sample, the method comprising: (a) preparing a binding mixture comprising —the nucleic acid containing sample, —a poly(alkylene oxide) polymer and —a salt and binding nucleic acid molecules of different sizes to a solid phase which comprises a functional group, preferably carboxylated magnetic particles; (b) separating the solid phase with the bound nucleic acid molecules from the remaining sample; and (c) contacting the solid phase with the bound nucleic acid molecules at least once with an elution composition comprising a poly(alkylene oxide) polymer and a salt to selectively elute nucleic acid molecules having a length below the cut-off value from the solid phase while larger nucleic acid molecules having a length above the cut-off value remain bound to the solid phase, wherein the concentration (w/v) of the poly(alkylene oxide) polymer in the elution composition is lower than the concentration (w/v) of the poly(alkylene oxide) polymer in the binding mixture of (a); (d) separating the solid phase with the bound larger nucleic acid molecules from the eluted nucleic acid molecules; and (e) optionally further purifying the eluted nucleic acid molecules. The method is particularly useful for separating extracellular target nucleic acids by size.
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