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235 条记录 (耗时 0.027 秒)

    Method for isolating extracellular nucleic acids using anion exchange particles
    1.
    发明授权
    Method for isolating extracellular nucleic acids using anion exchange particles 有权

    公开(公告)号：US12031125B2

    公开(公告)日：2024-07-09

    申请号：US17385460

    申请日：2021-07-26

    申请人： QIAGEN GmbH

    发明人： Alexander Wolf ,  Sandra Hammerschmidt ,  Thorsten Voss

    IPC分类号： C12N15/10 ,  C12Q1/6806

    CPC分类号： C12N15/101 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

    摘要： The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. between different lots/batches of the anion exchange particles and/or during storage of said particles. Moreover, including a polyoxyalkylene fatty alcohol ether in the binding mixture resulted in a higher purity of the obtained eluates revealing significantly less inhibition in a downstream reaction such as a PCR reaction.

    Method for analyzing aurka expression
    2.
    发明授权
    Method for analyzing aurka expression 有权

    公开(公告)号：US11840734B2

    公开(公告)日：2023-12-12

    申请号：US16494949

    申请日：2018-03-01

    申请人： Qiagen GmbH

    发明人： Siegfried Hauch ,  Markus Sprenger-Haussels

    IPC分类号： C12Q1/6886

    CPC分类号： C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/158

    摘要： Provided is a method for analysing the expression of one or more biomarker RNA molecules, comprising (A) isolating RNA from circulating tumor cells obtained from a subject, determining the expression of at least one biomarker RNA molecule in the isolated RNA and providing an expression profile based on the results; (B) isolating RNA from extracellular vesicles obtained from the subject, determining the expression of at least one biomarker RNA molecule in the isolated RNA and providing an expression profile based on the results; and (C) using the expression profiles determined in (A) and determined in (B) for a combined analysis of the results. Such combined analysis of the CTC and EV expression profiles enhances the prognostic and predictive value of the obtained results and can provide valuable diagnostic, prognostic and/or predictive information. The present method can thus be used as improved diagnostic, prognostic and/or predictive aid in the management of cancer patients. It can be used to support the diagnosis, prognosis or to choose the most appropriate treatment for cancer patients.

    Method for generating a RNA-sequencing library
    7.
    发明授权
    Method for generating a RNA-sequencing library 有权

    公开(公告)号：US11248262B2

    公开(公告)日：2022-02-15

    申请号：US15750345

    申请日：2016-08-24

    申请人： Qiagen GmbH

    发明人： Nan Fang ,  Bernhard Noll ,  Katja Heitz

    IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6855

    摘要： The invention refers to a novel method of preparing strand-specific RNA-sequencing libraries that can be used to identify DNA coding and non-coding strands that are transcribed to RNA. Such strand-specific RNA-sequencing libraries are especially useful in discovering anti-sense RNA and non-coding RNA. Random primer oligonucleotides, covalently coupled to a moiety, which blocks ligation, are used for RT reaction or the subsequent generation of the second DNA strand so that only one strand of the generated double-stranded DNA is ligated to sequencing adapters at the 5′ nucleotide and sequenced by paired-end sequencing.

    KIT FOR DETERMINING NUCLEIC ACID DEGRADATION
    8.
    发明申请
    KIT FOR DETERMINING NUCLEIC ACID DEGRADATION 有权

    公开(公告)号：US20220025451A1

    公开(公告)日：2022-01-27

    申请号：US17500204

    申请日：2021-10-13

    申请人： QIAGEN GmbH

    发明人： Miroslav VRANES ,  Ralf PEIST ,  Mario SCHERER ,  Stefan Otto CORNELIUS

    IPC分类号： C12Q1/6851

    摘要： A kit for use in assessing the status of nucleic acid degradation and/or the integrity of one or more nucleic acids in a sample by amplifying at least two overlapping regions within at least one locus and detecting the amount of the at least two amplification products. The kit includes a primer and at least two probes that bind under stringent conditions to a sequence that shares at least 80% sequence identity to a sequence selected from the group of sequences consisting of SEQ ID NO. 6 to SEQ ID NO. 47 over a stretch of 80 base pairs, or to a reverse complement thereof. One of the at least two probes binds to one of the at least two overlapping regions and the other of the at least two probes binds to a non-overlapping region.

    Cross Talk Compensation
    9.
    发明申请
    Cross Talk Compensation 有权

    公开(公告)号：US20210390666A1

    公开(公告)日：2021-12-16

    申请号：US17215956

    申请日：2021-03-29

    申请人： QIAGEN GmbH

    发明人： Thorsten ZERFASS ,  Maiko LOHEL ,  Fernando Carrillo OESTERREICH

    IPC分类号： G06T5/00 ,  G16B30/00 ,  G01N21/64 ,  G06T7/00 ,  G16B40/00 ,  G16B40/10

    摘要： Method and system for compensating intensity biases in a plurality of digital images. Each digital image of the plurality of digital images contains a plurality of objects and each of the plurality of objects is configured to receive at least one molecule comprising genetic information, wherein the at least one molecule is configured to receive one of at least a first fluorescent compound and a second fluorescent compound. A first digital image of the plurality of digital images is taken by an optical imaging system during emission of electromagnetic radiation by the first fluorescent compound, and a second digital image of the plurality of digital images is taken by the optical imaging system during emission of electromagnetic radiation by the second fluorescent compound.

    FIXATION OF A BIOLOGICAL MATERIAL
    10.
    发明申请
    FIXATION OF A BIOLOGICAL MATERIAL 有权

    公开(公告)号：US20210389216A1

    公开(公告)日：2021-12-16

    申请号：US17411667

    申请日：2021-08-25

    申请人： QIAGEN GmbH

    发明人： Christian Lenz ,  Daniel Grölz ,  Uwe Oelmüller

    IPC分类号： G01N1/30

    摘要： The present invention relates to a method for the treatment of a biological material, comprising the steps i) providing a biological material, and ii) contacting the biological material with a first non-aqueous composition comprising: (a1) 10 to 90 vol. % methanol, and (a2) at least one additional additive, and (a3) optionally an acid. iii) transferring the biological material into a second composition (B) comprising up to 99 vol. % ethanol as well as to a new composition for preservation of a biological material usable in said method and the biological material resulting from this method, a method for the analysis of a treated biological material, various kits as well as the use of the composition in such a method.