公开(公告)号：US20190093117A1

公开(公告)日：2019-03-28

申请号：US16048585

申请日：2018-07-30

申请人： R. J. Reynolds Tobacco Company

发明人： Xingpeng Li ,  Yijian He ,  Gregory P. Pogue ,  Emmett Ernest Hiatt ,  Darlene Madeline Lawson

IPC分类号： C12N15/82 ,  C12N9/22 ,  C12N15/11

摘要： The present disclosure provides compositions and methods for editing a target site of a plant genome by delivery of functional editing components using modified tobacco mosaic virus (mTMV). The methods disclosed herein can be used to deliver a gene editing system, such as a DNA endonuclease, to a tobacco plant cell for modification of a target site of the plant genome. Further, the methods and compositions disclosed herein provide for production of a RNA molecule encoding a meganuclease in vitro prior to delivery of the RNA to a plant cell. After introduction of the nucleic acid molecule encoding a functional editing component and subsequent expression of the functional editing components, the plant can be cultured and allowed to produce seeds having an edit at a genomic target site. The seeds can then undergo embryo rescue and be cultured to produce a modified plant without heterologous genetic material.