公开(公告)号：US20120159671A1

公开(公告)日：2012-06-21

申请号：US13114656

申请日：2011-05-24

Applicant: RODERICK SCOTT

Inventor： RODERICK SCOTT

IPC: C12N15/82

CPC classification number: C12N15/8261 ,  A01H5/10 ,  C07K14/415 ,  C12N15/823 ,  C12N15/8234 ,  Y02A40/146

Abstract: This invention relates to a method for modifying cell proliferation in a plant which comprises the step of transforming a plant, or plant propagating material, with a nucleic acid molecule comprising at least one regulatory sequence capable of directing expression within the integuments and/or seed coat and at least one nucleic acid sequence whose expression or transcription product is capable of directly or indirectly modulating cell proliferation, whereby on expression of that nucleic acid sequence cell proliferation is modified. The invention also relates to a plant which includes a nucleic acid molecule comprising at least one regulatory sequence capable of directing expression within integuments and/or seed coat an at least one nucleic acid sequence whose expression or transcription product is capable of directly or indirectly modulated cell proliferation, whereby on expression of that nucleic acid sequence cell proliferation is modified. The invention also relates to reproducible plant material including a nucleic acid molecule comprising at least one regulatory sequence capable of directing expression within integuments and/or seed coat and at least one nucleic acid sequence whose expression or transcription product is capable of directly or indirectly modulated cell proliferation, whereby expression of that nucleic acid sequence cell proliferation is modified. According to another aspect of the invention, there is provided a method for modifying cell proliferation in a plant which comprises the step of modulating the response of the plant to an auxin in which cell proliferation is modified to produce larger or smaller seeds than wild-type.

Abstract translation: 本发明涉及一种用于修饰植物细胞增殖的方法，该方法包括用包含至少一个能够在皮肤和/或种皮中表达的调控序列的核酸分子转化植物或植物繁殖材料的步骤 和至少一个其表达或转录产物能够直接或间接调节细胞增殖的核酸序列，从而在该核酸序列的表达细胞增殖被修饰。 本发明还涉及一种植物，其包含核酸分子，所述核酸分子包含至少一个能够引导表皮内和/或种皮的调节序列，所述调节序列的表达或转录产物能够直接或间接调节细胞的至少一个核酸序列 增殖，由此在该核酸序列的表达细胞增殖被修饰。 本发明还涉及可再现的植物材料，其包括核酸分子，其包含至少一个能够引导表皮和/或种皮中的表达的调节序列和至少一个其表达或转录产物能够直接或间接调节细胞的核酸序列 从而改变该核酸序列细胞增殖的表达。 根据本发明的另一方面，提供了一种修饰植物细胞增殖的方法，其包括调节植物对生长素的反应的步骤，其中细胞增殖被修饰以产生比野生型更大或更小的种子 。