公开(公告)号：US20230265378A1

公开(公告)日：2023-08-24

申请号：US18041238

申请日：2021-08-10

Applicant: ROYAL MELBOURNE INSTITUTE OF TECHNOLOGY

Inventor： Lizebona August AMBATTU ,  Shwathy RAMESAN ,  Leslie YEO

IPC: C12M1/42 ,  C12M1/32 ,  A61K9/50 ,  C12N5/09

CPC classification number: C12M35/04 ,  C12M23/12 ,  A61K9/5068 ,  C12N5/0693 ,  C12N2521/10

Abstract: Embodiments relate to a method and apparatus for stimulating the production of extracellular vesicles (EVs) from a population of cells. Some embodiments relate to a method for stimulating the production of extracellular vesicles (EVs) from a population of cells, the method comprising: (i) exposing a culture media comprising the population of cells to acoustic wave energy and (ii) harvesting the EVs produced from the population of cells following the exposure. Some embodiments relate to an apparatus for use in stimulating the production of EVs from a population of cells, the apparatus comprising: (i) an acoustic wave generator configured to generate acoustic energy at a selected power and frequency; and (ii) a receptacle for accommodating a population of cells in a culture media, the receptacle being configured to receive acoustic energy generated by the acoustic wave generator.