公开(公告)号：US20230265378A1

公开(公告)日：2023-08-24

申请号：US18041238

申请日：2021-08-10

Applicant: ROYAL MELBOURNE INSTITUTE OF TECHNOLOGY

Inventor： Lizebona August AMBATTU ,  Shwathy RAMESAN ,  Leslie YEO

IPC: C12M1/42 ,  C12M1/32 ,  A61K9/50 ,  C12N5/09

CPC classification number: C12M35/04 ,  C12M23/12 ,  A61K9/5068 ,  C12N5/0693 ,  C12N2521/10

Abstract: Embodiments relate to a method and apparatus for stimulating the production of extracellular vesicles (EVs) from a population of cells. Some embodiments relate to a method for stimulating the production of extracellular vesicles (EVs) from a population of cells, the method comprising: (i) exposing a culture media comprising the population of cells to acoustic wave energy and (ii) harvesting the EVs produced from the population of cells following the exposure. Some embodiments relate to an apparatus for use in stimulating the production of EVs from a population of cells, the apparatus comprising: (i) an acoustic wave generator configured to generate acoustic energy at a selected power and frequency; and (ii) a receptacle for accommodating a population of cells in a culture media, the receptacle being configured to receive acoustic energy generated by the acoustic wave generator.

公开(公告)号：US20220401662A1

公开(公告)日：2022-12-22

申请号：US17766446

申请日：2020-10-05

Applicant: Royal Melbourne Institute of Technology

Inventor： Leslie YEO ,  Amgad REZK ,  Amarin MCDONNELL ,  Callum KNIGHT

IPC: A61M11/00 ,  B05B17/06

Abstract: A nebuliser for nebulising liquid droplets, including: a housing; at least one piezoelectric substrate (2) accommodated within the housing and having a transducer surface (2a) upon which is located at least one electroacoustic transducer (48) for generating acoustic wave energy within the at least one piezoelectric substrate (2), and an opposing non-transducer surface (2b); a liquid supply system for supplying a liquid to at least one of the transducer and non-transducer surfaces, the liquid supply system including a reservoir (3) for accommodating the liquid, and at least one relatively rigid supply conduit in contact with the at least one piezoelectric substrate (2) for supplying the liquid from the reservoir (3) to the at least one piezoelectric substrate (2); and a sensor for detecting a volume of liquid on the at least one piezoelectric substrate (2).

公开(公告)号：US20160301381A1

公开(公告)日：2016-10-13

申请号：US15029433

申请日：2014-09-30

Applicant: ROYAL MELBOURNE INSTITUTE OF TECHNOLOGY

Inventor： Amgad REZK ,  Leslie YEO ,  James FRIEND

IPC: H03H9/25 ,  H02N2/16 ,  B01L3/00 ,  B05B17/06

CPC classification number: H03H9/25 ,  B01L3/50273 ,  B01L2300/12 ,  B01L2400/0415 ,  B05B17/0661 ,  B05B17/0684 ,  H01L41/047 ,  H02N2/16

Abstract: A piezoelectric actuation platform (1) including piezoelectric substrate (3) formed from a single crystal piezoelectric material, and at least one simple electrode (5) in contact with the piezoelectric substrate for applying an electrical signal to the substrate such that a lamb or surface acoustic wave can be generated within said substrate.

Abstract translation: 一种包括由单晶压电材料形成的压电衬底（3）的压电致动平台（1）和至少一个与所述压电衬底接触的简单电极（5），用于向所述衬底施加电信号，使得羊肉或表面 可以在所述衬底内产生声波。