公开(公告)号：US07108870B2

公开(公告)日：2006-09-19

申请号：US10822858

申请日：2004-04-13

申请人： Rajender Singh Sangwan ,  Narayan Das Chaurasiya ,  Laxmi Narayan Misra ,  Payare Lal ,  Girish Chandra Uniyal ,  Neelam Singh Sangwan ,  Avdhesh Kumar Srivastava ,  Krishan Avtar Suri ,  Ghulam Nabi Qazi ,  Rakesh Tuli

发明人： Rajender Singh Sangwan ,  Narayan Das Chaurasiya ,  Laxmi Narayan Misra ,  Payare Lal ,  Girish Chandra Uniyal ,  Neelam Singh Sangwan ,  Avdhesh Kumar Srivastava ,  Krishan Avtar Suri ,  Ghulam Nabi Qazi ,  Rakesh Tuli

IPC分类号： A61K36/00 ,  A61K31/35

CPC分类号： A61K36/81

摘要： The present invention relates to an improved process of analytical and quantitative isolation of withaferin-A from Withania somnifera (Sanskrit: Ashwagandha, English: winter cherry) and other plants and products therefrom, said method comprising steps of selecting most appropriate extraction medium composition, with extraction solvent system consisting of a defined admixture of water and alcohol in a range of proportion of alcohol within a narrow range of (methanol, ethanol etc.) (preferably 60% alcohol and 40% water) for the dry plant material and from 60% to 80% alcohol in the alcohol-water mixture for fresh plant material, the invented extractant composition leading to better/improved yields and accurate quantitative estimations of withaferin A existent in planta or in materia, selecting effective state of the tissue particularly use of the fresh materials improving yields, providing logistic benefits of isolations and diagnosis, and according several other advantages including technological, economic, preventing contaminations etc., the said method being valuable and advantageous for standardization of botanicals, metabolomic characterization of plants particularly chemotypes, analyzing metabolic transitions under physiological, genetical, environmental and biotic perturbations.

摘要翻译： 本发明涉及一种分离和定量分离来自隐翅藻（梵语：Ashwagandha，英语：冬樱桃）和其他植物及其产物的咖啡因-A的改进方法，所述方法包括以下步骤：选择最合适的提取培养基组合物， 萃取溶剂体系，由干燥植物材料的（甲醇，乙醇等）（优选60％酒精和40％水）的窄范围内的醇的比例范围内的水和醇的混合物组成， 到新鲜植物材料的酒精 - 水混合物中的80％酒精，本发明的提取剂组合物导致在植物或物质中存在的更好/提高的产量和准确的定量估计，选择组织的有效状态，特别是使用新鲜的 材料提高产量，提供隔离和诊断的后勤优势，以及其他几个优势，包括技术 所述方法对于植物学的标准化，植物的代谢组学特征，特别是化学型，分析生理，遗传，环境和生物扰动下的代谢转变是有价值和有利的。