公开(公告)号：US09075962B1

公开(公告)日：2015-07-07

申请号：US14177312

申请日：2014-02-11

申请人： Raymond Lau ,  R. Paul Johnson

发明人： Raymond Lau ,  R. Paul Johnson

IPC分类号： H04L29/06 ,  G06F21/10 ,  H04L9/32

CPC分类号： G06F21/10 ,  H04L9/3247 ,  H04L63/123 ,  H04L63/168 ,  H04L65/4084 ,  H04L65/608 ,  H04L67/02 ,  H04N21/2347 ,  H04N21/2541 ,  H04N21/4405

摘要： Methods and apparatus, including computer program products, for secure video delivery. A method includes providing a cryptographically signed manifest Uniform Resource Locator (URL) to the Hypertext Transfer Protocol (HTTP) manifest format player, the signed URL including at least a validity time span and an identification of a video, in the manifest format player, loading the manifest URL, the manifest URL pointing to an instantiation of a proxy, in the proxy, loading the manifest URL and verifying a validity of the signed URL, in the proxy, retrieving the manifest URL contents, in the proxy, substituting each non-metadata entry in the manifest URL contents with signed URLs either to an underlying video segment or back to an instantiation of the proxy if an entry another manifest, and in the HTTP manifest player, processing the manifest altered with signed URLs as a standard manifest, playing the content.

摘要翻译： 方法和设备，包括计算机程序产品，用于安全的视频传送。 一种方法包括向超文本传输​​协议（HTTP）清单格式播放器提供加密签名的清单统一资源定位符（URL），该签名URL至少包括有效时间段和清单格式播放器中的视频标识，加载 代理中的清单URL，指示代理的实例的清单URL，代理中的加载清单URL并验证签名的URL的有效性，在代理中检索清单URL内容， 在具有签名的URL的清单URL内容中的元数据条目到底层视频段或者如果条目另一清单返回到代理的实例，并且在HTTP清单播放器中处理该清单作为标准清单改变的标签清单中的元数据条目 内容。

公开(公告)号：US5677139A

公开(公告)日：1997-10-14

申请号：US426782

申请日：1995-04-21

申请人： R. Paul Johnson ,  Michael Rosenzweig ,  David T. Scadden

发明人： R. Paul Johnson ,  Michael Rosenzweig ,  David T. Scadden

IPC分类号： C12N5/02 ,  C12N5/0783 ,  G01N33/50 ,  C12Q1/02

CPC分类号： C12N5/0636 ,  G01N33/5005 ,  C12N2501/23 ,  C12N2502/11 ,  C12N2503/00

摘要： The invention involves a method for the in vitro T cell production. A monolayer of non-human primate thymic stromal cells are cocultured in vitro with primate hematopoietic T cell progenitor cells. This results in the differentiation and growth of mature T cells. The T cells may be isolated at any sequential stage of differentiation and further expanded by coculture with a mitogenic agent. The T cells also may be genetically altered at any stage of the process. The effect of agents on the growth and differentiation of T cells may be measured by comparing a coculture containing the agent with a control coculture and comparing the differentiation or growth of the T cells progenitor cells in the test culture with the control culture. Kits and novel populations of T cells are provided.

摘要翻译： 本发明涉及体外T细胞生产的方法。 单层非人灵长类动物胸腺基质细胞在体外与灵长类动物造血T细胞祖细胞共培养。 这导致成熟T细胞的分化和生长。 可以在分化的任何连续阶段分离T细胞，并通过与促有丝分裂剂共同培养进一步扩增。 T细胞也可以在该过程的任何阶段进行遗传改变。 试剂对T细胞生长和分化的影响可以通过将含有该试剂的共培养物与对照共培养进行比较来测量，并将测试培养物中T细胞祖细胞的分化或生长与对照培养物进行比较。 提供试剂盒和新型T细胞群体。