公开(公告)号：US06818446B2

公开(公告)日：2004-11-16

申请号：US09990613

申请日：2001-11-21

Applicant: Reen Wu ,  Yin Chen

Inventor： Reen Wu ,  Yin Chen

IPC: C12N1574

CPC classification number: C07K14/4727

Abstract: The invention relates to compositions and methods for the assessment of mucin gene expression. The invention also relates to compositions and methods for the identification of compounds useful in the treatment of various disorders caused by mucin overproduction. The invention provides novel MUC5B promoter sequences and reporter constructs comprising these MUC5B promoter sequences. The invention further provides methods for drug screening to identify compounds have the ability to inhibit MUC5B gene expression. Compounds having the ability to inhibit MUC5B gene expression find use in the treatment of diseases characterized by mucin hyperproduction.

Abstract translation: 本发明涉及用于评估粘蛋白基因表达的组合物和方法。 本发明还涉及用于鉴定可用于治疗由粘蛋白过量产生引起的各种疾病的化合物的组合物和方法。 本发明提供新的MUC5B启动子序列和包含这些MUC5B启动子序列的报道构建体。 本发明进一步提供用于药物筛选以鉴定化合物具有抑制MUC5B基因表达的能力的方法。 具有抑制MUC5B基因表达能力的化合物可用于治疗以粘蛋白过量产生为特征的疾病。

公开(公告)号：US06218114B1

公开(公告)日：2001-04-17

申请号：US09049569

申请日：1998-03-27

Applicant: Konan Peck ,  Jeremy J. W. Chen ,  Pan-Chyr Yang ,  Reen Wu ,  Fu Chang ,  Yi-Wen Chu ,  Cheng-Wen Wu

Inventor： Konan Peck ,  Jeremy J. W. Chen ,  Pan-Chyr Yang ,  Reen Wu ,  Fu Chang ,  Yi-Wen Chu ,  Cheng-Wen Wu

IPC: C12Q168

CPC classification number: C12Q1/6809

Abstract: The invention relates to a method of detecting a differentially expressed gene in a first sample of nucleic acids representing a first population of RNA transcripts and a second sample of nucleic acids representing a second population of RNA transcripts. The nucleic acids in the samples are labled with a member of specific binding pair, and the labeled nucleic acids in each sample are then hybridized to an excess of copies of a gene-specific sequence. The hybridized nucleic acids in each sample are further labeled by binding a second member of the specific binding pair to the first member, in which the second member has an activity to convert a chromogenic substrate into a chromogen. As a result of contacting the second member with the chromogenic substrate, the chromogenic substrate is converted into the chromogen. A difference in the amounts of chromogen produced from assaying the two samples indicate that the gene-specific sequence is differentially expressed in the samples.

Abstract translation: 本发明涉及检测代表第一RNA转录物群体的核酸的第一样本中的差异表达基因的方法和表示第二RNA转录物群体的第二核酸样品。 样品中的核酸用特异性结合对的成员进行标记，然后将每个样品中的标记的核酸与过量的基因特异性序列的拷贝杂交。 通过将特异性结合对的第二成员与第一成员结合来进一步标记每个样品中的杂交核酸，其中第二成员具有将显色底物转化为色原体的活性。 作为使第二构件与显色底物接触的结果，将显色底物转化为色原体。 通过测定两个样品产生的色原体的量的差异表明基因特异性序列在样品中差异表达。