公开(公告)号：US20240192229A1

公开(公告)日：2024-06-13

申请号：US18286682

申请日：2022-04-15

Applicant: Regents of the University of Minnesota

Inventor： Peter C. Christenson ,  Gage Rowden ,  Sang-Hyun Oh ,  Peter A. Larsen ,  Manci Li

IPC: G01N33/68 ,  G01N33/58

CPC classification number: G01N33/6896 ,  G01N33/587 ,  G01N2800/2821 ,  G01N2800/2828 ,  G01N2800/2835

Abstract: This document relates to methods and materials for detecting the presence or absence of misfolded polypeptides in a sample. For example, methods and materials for amplifying a sample (e.g., a biological sample or an environmental sample) such that misfolded polypeptides present in the sample can aggregate to form fibrils and/or globular polypeptide aggregates and contacting the amplified sample with a solution containing metal nanoparticles (e.g., gold nanoparticles) or one or more organic dyes (e.g., Congo Red) to detect the presence or absence of fibrils and/or globular polypeptide aggregates are provided. In some cases, methods and materials for determining if a mammal (e.g., a human) has a proteinopathy based, at least in part, in the presence or absence of misfolded polypeptides in a sample obtained from the mammal are provided.

公开(公告)号：US20240264176A1

公开(公告)日：2024-08-08

申请号：US18567172

申请日：2022-06-06

Applicant: Regents of the University of Minnesota ,  Creighton University

Inventor： Peter A. Larsen ,  Tiffany M. Wolf ,  Marc D. Schwabenlander ,  Jason C. Bartz ,  Qi Yuan

IPC: G01N33/68 ,  G01N1/40

CPC classification number: G01N33/6896 ,  G01N1/4077 ,  G01N2001/4094 ,  G01N2800/2828

Abstract: This document provides methods and materials for detecting misfolded poly peptides. For example, devices (e.g., feeding devices) designed to collect samples from one or more mammals (e.g., one or more cervids) are provided. In some cases, methods and materials that can be used to detect one or more prion diseases (e.g., chronic wasting disease (CWD)) in a population of mammals (e.g., a herd of cervids) based, at least in part, on the presence or absence of misfolded polypeptides in a sample from one or more mammals that was obtained from a feeding device are provided.