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1.发明授权公开(公告)号：US07816080B2
公开(公告)日：2010-10-19
申请号：US11605049
申请日：2006-11-27
CPC分类号： C12Q1/686 , C12Q1/6888 , C12Q1/6895 , C12Q2600/156
摘要： The present invention provides novel methods for characterizing organisms by identifying the presence, absence, size or sequence polymorphism of intronic regions. The method involves selecting intronic regions from nuclear or organellar gene sequences that are useful for differentiating between and among taxonomic groupings of organisms. Such intronic regions can be analyzed directly or after amplification in a primer extension reaction. The amplification product is then analyzed by, for example, size fractionation, nucleotide sequencing or (RFLP). Intronic regions that contain an open reading frame encoding all or a portion of a protein can be used to generate antibodies to detect the presence or absence of the protein, which indicates the presence or absence of the intronic region. Methods of detecting an organism in a sample by detecting the presence or absence of one or more intronic regions also are provided using nucleic acid based or immunological based approaches. Kits are provided for practicing the methods of the invention.
摘要翻译： 本发明通过鉴定内含子区域的存在，不存在，大小或序列多态性来提供用于表征生物体的新方法。 该方法包括从核或细胞器基因序列中选择可用于区分生物分类组之间和之间的内含子区域。 这样的内含子区域可以在引物延伸反应中直接分析或扩增后进行分析。 然后通过例如大小分级，核苷酸测序或（RFLP）分析扩增产物。 含有编码蛋白质全部或部分的开放阅读框的内含子区域可用于产生抗体以检测蛋白质的存在或不存在，其指示内含子区域的存在或不存在。 通过检测一个或多个内含子区域的存在或不存在来检测样品中生物体的方法也使用基于核酸或基于免疫学的方法提供。 提供了用于实施本发明方法的试剂盒。
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公开(公告)号：US06599701B1
公开(公告)日：2003-07-29
申请号：US09645055
申请日：2000-08-23
IPC分类号： C12Q168
CPC分类号： C12Q1/686 , C12Q1/6888 , C12Q1/6895 , C12Q2600/156
摘要： The present invention provides novel methods for characterizing organisms by identifying the presence, absence, size or sequence polymorphism of intronic regions. The method involves selecting intronic regions from nuclear or organellar gene sequences that are useful for differentiating between and among taxonomic groupings of organisms. Such intronic regions can be analyzed directly or after amplification in a primer extension reaction. The amplification product is then analyzed by, for example, size fractionation, nucleotide sequencing or (RFLP). Intronic regions that contain an open reading frame encoding all or a portion of a protein can be used to generate antibodies to detect the presence or absence of the protein, which indicates the presence or absence of the intronic region. Methods of detecting an organism in a sample by detecting the presence or absence of one or more intronic regions also are provided using nucleic acid based or immunological based approaches. Kits are provided for practicing the methods of the invention.
摘要翻译： 本发明通过鉴定内含子区域的存在，不存在，大小或序列多态性来提供用于表征生物体的新方法。 该方法包括从核或细胞器基因序列中选择可用于区分生物分类组之间和之间的内含子区域。 这样的内含子区域可以在引物延伸反应中直接分析或扩增后进行分析。 然后通过例如大小分级，核苷酸测序或（RFLP）分析扩增产物。 含有编码蛋白质全部或部分的开放阅读框的内含子区域可用于产生抗体以检测蛋白质的存在或不存在，其指示内含子区域的存在或不存在。 通过检测一个或多个内含子区域的存在或不存在来检测样品中生物体的方法也使用基于核酸或基于免疫学的方法提供。 提供了用于实施本发明方法的试剂盒。
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3.发明申请Methods of making and uses of compositions that modulate intronic region-encoded protein function 审中-公开制备和使用调节内含子区域编码的蛋白质功能的组合物的方法公开(公告)号：US20090215668A1
公开(公告)日：2009-08-27
申请号：US12288618
申请日：2008-10-22
申请人： Rhonda J. Honeycutt , David Ralph
发明人： Rhonda J. Honeycutt , David Ralph
CPC分类号： C12Q1/689 , C07K14/37 , C12Q1/6895 , C12Q2600/156
摘要： This invention relates to compositions and methods for modulating cellular activity of non-human organisms, and in particular fungi, and methods of identifying and using antifungal agents with improved specificity. More particularly, the technology described herein relates to the identification and use of compounds that target intron-encoded proteins, such as maturases.
摘要翻译： 本发明涉及用于调节非人生物体，特别是真菌的细胞活性的组合物和方法，以及鉴定和使用具有改善的特异性的抗真菌剂的方法。 更具体地，本文描述的技术涉及鉴定和使用靶向内含子编码的蛋白质（例如成熟酶）的化合物。
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