公开(公告)号：US06475768B1

公开(公告)日：2002-11-05

申请号：US09545569

申请日：2000-04-07

申请人： Ricardo Román Cordero Otero ,  Márk Gárdonyi ,  Bärbel Hahn-Hägerdal ,  Willem Heber van Zyl ,  Eva Anna Viktoria Dackehag

发明人： Ricardo Román Cordero Otero ,  Márk Gárdonyi ,  Bärbel Hahn-Hägerdal ,  Willem Heber van Zyl ,  Eva Anna Viktoria Dackehag

IPC分类号： C12N990

CPC分类号： C12Y503/01005 ,  C12N9/92

摘要： The present invention relates to novel nucleic acid sequences which, upon expression in a procaryotic or eucaryotic cell, code for a polypeptide having an elevated specific xylose isomerase activity compared to the wildtype Thermus thermophilus xylose isomerase. They are selected from a) nucleic acid sequences shown in SEQ. ID. No 1; b) the complementary strand of the sequence defined in (a) above; c) nucleic acid sequences which hybridize to the sequences defined in (a) or (b) above; d) nucleic acid sequences which, but for the degeneracy of the genetic code, would hybridize to the sequences defined in (a), (b) or (c) above and which code for the same polypeptide as those defined in (a), (b) or (c) above. The present invention further provides a process of producing ethanol from xylose containing materials comprising contacting cells that express such nucleic acid sequences and novel modified xylose isomerases that advantageously can be applied in the production of fructose syrups.

摘要翻译： 本发明涉及新颖的核酸序列，其在原核或真核细胞中表达时编码与野生型嗜热栖热菌嗜热木糖木糖异构酶相比具有升高的特异性木糖异构酶活性的多肽。 它们选自a）SEQ ID NO： ID。 没有1; b）上文（a）中定义的序列的互补链; c）与上述（a）或（b）中定义的序列杂交的核酸序列; d）核酸序列，但是对于遗传密码的简并性，其将与上述（a），（b）或（c）中定义的序列杂交，并且与（a）中定义的相同多肽的编码， （b）或（c）。 本发明进一步提供了一种从含有木糖的材料生产乙醇的方法，包括使表达这种核酸序列的细胞接触和有利地可用于果糖糖浆生产中的新型修饰的木糖异构酶。