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    1-deoxy-D-xylulose-5-phosphate reductoisomerases, and methods of use
    2.
    发明授权
    1-deoxy-D-xylulose-5-phosphate reductoisomerases, and methods of use 有权
    1-脱氧-D-木酮糖-5-磷酸还原异构酶及其使用方法

    公开(公告)号：US06420159B2

    公开(公告)日：2002-07-16

    申请号：US09874562

    申请日：2001-06-04

    申请人： Rodney B. Croteau ,  Bernd M. Lange

    发明人： Rodney B. Croteau ,  Bernd M. Lange

    IPC分类号： C12N990

    CPC分类号： C12N9/0006

    摘要： The present invention relates to isolated DNA sequences which code for the expression of plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein, such as the sequence presented in SEQ ID NO:1 which encodes a 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein from peppermint (Mentha x piperita). Additionally, the present invention relates to isolated plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase, to modified host cells transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence of the invention.

    摘要翻译： 本发明涉及编码植物1-脱氧-SMALLCAPS> D D - 5-羟基-5-磷酸还原异构酶蛋白。 此外，本发明涉及分离的植物1-脱氧-β-氧化物-5-磷酸还原异构酶蛋白。 在其它方面，本发明涉及可复制的重组克隆载体，其包含编码植物1-脱氧-SMALLCAPS> D / /SMALLCAPSα-羟基-5-磷酸还原异构酶的核酸序列，转化的修饰的宿主细胞， 转染，感染和/或注射本发明的重组克隆载体和/或DNA序列。

    Polypeptides having glucose isomerase activity and nucleic acids encoding same
    3.
    发明授权
    Polypeptides having glucose isomerase activity and nucleic acids encoding same 失效
    具有葡萄糖异构酶活性的多肽和编码其的核酸

    公开(公告)号：US06372476B1

    公开(公告)日：2002-04-16

    申请号：US09383318

    申请日：1999-08-26

    申请人： Karima Srih Belguith ,  Radhouane Ellouz ,  Samir Bejar

    发明人： Karima Srih Belguith ,  Radhouane Ellouz ,  Samir Bejar

    IPC分类号： C12N990

    CPC分类号： C12N9/92 ,  C12Y503/01005

    摘要： Disclosed are isolated polypeptides having glucose isomerase activity selected from: (a) a polypeptide having an amino acid sequence which has at least 95% identity with amino acids of SEQ ID NO:2; (b) a variant of the polypeptide having an amino acid sequence of SEQ ID NO:2 comprising a substitution, deletion, and/or insertion of one or more amino acids; (c) a fragment of (a) that has glucose isomerase activity; and (d) a polypeptide having a pH optimum in the range of 5.7 to 6.3 at 60° C., a pH optimum in the range of 6.1 to 6.7 at 90° C and a temperature optimum of above 90° C. Also disclosed are isolated nucleic acid sequences encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

    摘要翻译： 公开了具有选自以下的葡萄糖异构酶活性的分离的多肽：（a）具有与SEQ ID NO：2的氨基酸具有至少95％同一性的氨基酸序列的多肽;（b）具有氨基酸的多肽的变体 包含一个或多个氨基酸的取代，缺失和/或插入的SEQ ID NO：2的序列;（c）具有葡萄糖异构酶活性的（a）的片段; 和（d）在60℃下pH最佳为5.7至6.3的多肽，在90℃下最佳为6.1至6.7，最适温度为90℃以上的pH最佳。 分离的编码多肽的核酸序列，核酸构建体，载体和包含核酸序列的宿主细胞以及产生和使用多肽的方法。

    Polypeptides capable of interacting with the human topoisomerase III&agr;
    4.
    发明授权
    Polypeptides capable of interacting with the human topoisomerase III&agr; 失效
    能够与人拓扑异构酶IIIal相互作用的多肽

    公开(公告)号：US06706514B1

    公开(公告)日：2004-03-16

    申请号：US09856930

    申请日：2001-06-25

    申请人： Alain Fournier ,  Hélène Goulaouic ,  Jean-Francois Riou

    发明人： Alain Fournier ,  Hélène Goulaouic ,  Jean-Francois Riou

    IPC分类号： C12N990

    CPC分类号： C12N9/90 ,  A61K38/00

    摘要： The invention concerns novel polypeptides capable of interacting with the human topoisomerase III&agr; and the nucleic acid sequences coding for said polypeptides. The invention also concerns a method for identifying compounds capable of interacting with said polypeptides and a method for identifying molecules capable of modulating the interaction of the topoisomerase III&agr; with said polypeptides.

    摘要翻译： 本发明涉及能够与人拓扑异构酶IIIa相互作用的新型多肽和编码所述多肽的核酸序列。 本发明还涉及鉴定能够与所述多肽相互作用的化合物的方法，以及鉴定能够调节拓扑异构酶IIIal与所述多肽的相互作用的分子的方法。

    Phosphohexuloisomerase and gene therefor
    6.
    发明授权
    Phosphohexuloisomerase and gene therefor 失效
    磷酸己糖异构酶及其基因

    公开(公告)号：US06630341B2

    公开(公告)日：2003-10-07

    申请号：US09897956

    申请日：2001-07-05

    申请人： Nobuo Kato ,  Hisashi Yasueda

    发明人： Nobuo Kato ,  Hisashi Yasueda

    IPC分类号： C12N990

    CPC分类号： C12N9/90

    摘要： There are provided a DNA coding for phosphohexuloisomerase, which is a protein defined in the following (A) or (B), and a method for producing the enzyme: (A) a protein having the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing, (B) a protein having the amino acid sequence of SEQ ID NO: 3 shown in Sequence Listing including substitution, deletion, insertion or addition of one or several amino acid residues and having phosphohexulose isomerase activity.

    摘要翻译： 提供了编码以下（A）或（B）中定义的蛋白质的磷酸己糖异构酶的DNA及其制造方法：（A）具有SEQ ID NO：3的氨基酸序列的蛋白质 （B）具有序列表所示的氨基酸序列SEQ ID NO：3的蛋白质，其包含一个或几个氨基酸残基的取代，缺失，插入或添加并具有磷酸己糖糖异构酶活性。

    Hexulose phosphate isomerase gene
    7.
    发明授权
    Hexulose phosphate isomerase gene 失效
    己糖基磷酸异构酶基因

    公开(公告)号：US06331428B1

    公开(公告)日：2001-12-18

    申请号：US09185501

    申请日：1998-11-04

    申请人： Nobuo Kato

    发明人： Nobuo Kato

    IPC分类号： C12N990

    CPC分类号： C12N9/90

    摘要： Hexulose phosphate synthase was purified from Mycobacterium gastri to determine an amino acid sequence of the enzyme. Oligonucleotide primers to be used for PCR were synthesized on the basis of obtained amino acid sequence information. A DNA fragment, which was amplified by performing PCR by using a template of genomic DNA prepared from Mycobacterium gastri, was used as a probe so that colony hybridization was carried out with respect to a library comprising fragments obtained by digesting Mycobacterium gastri genomic DNA with PstI to obtain a positive clone. Thus, the DNA fragment which contains ORF encoding hexulose phosphate synthase was obtained. Furthermore, the DNA fragment cloned contained other ORF(ORF-1). The expression system of ORF-1 was constructed (pT-HPIS-1), and it was introduced into Escherichia coli cell. After the induction of the expression of ORF-1, the hexulose phosphate isomerase activity was found in the extract of cells harvoring pT-HPIS-1. Thus, gene, which coded for hexulose phosphate isomerase, was obtained together with ORF which coded for hexulose phosphate synthase.

    摘要翻译： 从胃分枝杆菌中纯化己糖磷酸合成酶以确定该酶的氨基酸序列。 基于获得的氨基酸序列信息合成用于PCR的寡核苷酸引物。 使用通过使用由分枝杆菌（Gastri）制备的基因组DNA的模板进行PCR扩增的DNA片段作为探针，以便对包含通过用PstI消化胃分枝杆菌基因组DNA获得的片段的文库进行集落杂交 以获得阳性克隆。 因此，获得含有编码磷酸内酯磷酸合成酶的ORF的DNA片段。 此外，克隆的DNA片段含有其他ORF（ORF-1）。 构建了ORF-1的表达系统（pT-HPIS-1），并将其导入大肠杆菌细胞。 在诱导ORF-1的表达后，在含有pT-HPIS-1的细胞提取物中发现己糖基磷酸异构酶活性。 因此，编码己糖基磷酸异构酶的基因与编码磷酸内酯磷酸合成酶的ORF一起获得。

    Tyrosine-containing cyclophilin and related methods
    10.
    发明授权
    Tyrosine-containing cyclophilin and related methods 失效
    含酪氨酸的亲环蛋白及相关方法

    公开(公告)号：US06649395B1

    公开(公告)日：2003-11-18

    申请号：US09715285

    申请日：2000-11-17

    申请人： Clotilde K. S. Carlow ,  Xiqiang Hong ,  Dong Ma

    发明人： Clotilde K. S. Carlow ,  Xiqiang Hong ,  Dong Ma

    IPC分类号： C12N990

    CPC分类号： C12N9/90 ,  C07K2319/00 ,  Y10S530/81

    摘要： The present invention relates to the use of these cyclophilins, hereinafter referred to as ‘tyrosine-containing’ cyclophilins, in a method for identifying compounds capable of binding to and/or inhibiting the enzymatic activity of these proteins. Such compounds may be further screened for their ability to inhibit parasites which are not susceptible to the anti-parasitic effects of CsA.

    摘要翻译： 本发明涉及这些亲环蛋白的用途，以下称为“含酪氨酸”的亲环蛋白，用于鉴定能够结合和/或抑制这些蛋白质的酶活性的化合物的方法。 可以对这些化合物进一步筛选其抑制对CsA的抗寄生作用不敏感的寄生虫的能力。