公开(公告)号：US5714340A

公开(公告)日：1998-02-03

申请号：US260939

申请日：1994-06-16

申请人： Richard Calvin Sutton ,  Margaret Jeanette Smith-Lewis ,  Linda Ann Mauck ,  Wayne Arthur Bowman ,  Susan Jean Danielson

发明人： Richard Calvin Sutton ,  Margaret Jeanette Smith-Lewis ,  Linda Ann Mauck ,  Wayne Arthur Bowman ,  Susan Jean Danielson

IPC分类号： G01N33/543 ,  G01N33/53 ,  G01N33/545

CPC分类号： G01N33/54386 ,  Y10S435/81 ,  Y10S436/81

摘要： A dry immunoassay analytical element, for assaying a ligand is disclosed. The element comprises, in the following order, (a) a layer containing a labeled ligand, (b) a bead spreading layer, c) a cross-linked hydrophilic polymer layer and d) a support; wherein (i) a fixed concentration of an immobilized receptor for the labeled ligand is located in a zone at the interface of layers (b) and (c); and (ii) the receptors are immobilized by being covalently bonded to polymeric beads that are smaller than the beads in layer (c).

摘要翻译： 公开了用于测定配体的干燥免疫测定分析元件。 该元件按以下顺序包含（a）含有标记配体的层，（b）珠扩散层，c）交联的亲水性聚合物层和d）载体; 其中（i）用于标记配体的固定化受体的固定浓度位于层（b）和（c）的界面处的区域中; 和（ii）通过共价结合到层（c）中小于珠粒的聚合物珠粒来固定受体。