公开(公告)号：US06303339B1

公开(公告)日：2001-10-16

申请号：US07576423

申请日：1991-05-24

申请人： Richard D. Sontheimer ,  Tsu-San Lieu ,  Daniel P. McCauliffe ,  J. Donald Capra

发明人： Richard D. Sontheimer ,  Tsu-San Lieu ,  Daniel P. McCauliffe ,  J. Donald Capra

IPC分类号： C12P2104

CPC分类号： G01N33/564 ,  C07K14/46 ,  C12Q1/6876 ,  G01N2800/24

摘要： The present disclosure relates to DNA sequences encoding one or more antigenic epitopes of the Ro 60 kD autoantigen, as well as to antigenic peptides themselves which correspond antigenically to epitopes found on the Ro/SS-A ribonucleoprotein (RNP) particle. Peptides which incorporate the antigenic epitopic core sequences disclosed herein may be employed in place of the Ro/SS-A RNP in any of a variety immunoassays including ELISA assays. The polypeptides of the invention may be employed in colorimetric assays for the identification and characterization of autoimmune diseases such as systemic lupus erythematosus (SLE) and Sjogren's syndrome. The DNA sequences disclosed herein may be employed in the preparation of the 60 kD Ro antigen, peptides which incorporate antigenic core sequences thereof, to probe for Ro sequences by hybridization analysis, and the like.

摘要翻译： 本公开内容涉及编码Ro 60kD自身抗原的一种或多种抗原表位的DNA序列，以及抗原肽本身对应于在Ro / SS-A核糖核蛋白（RNP）颗粒上发现的表位的抗原性肽。 可以使用包含本文公开的抗原性表位核心序列的肽来代替Ro / SS-A RNP，其包括ELISA测定中的任一种免疫测定。 本发明的多肽可用于鉴定和表征自身免疫疾病如系统性红斑狼疮（SLE）和干燥综合征的比色测定。 本文公开的DNA序列可以用于制备60kD Ro抗原，其中包含其抗原核心序列的肽，通过杂交分析来探测Ro序列等。