公开(公告)号：US20080019883A1

公开(公告)日：2008-01-24

申请号：US11669827

申请日：2007-01-31

申请人： Richard Fike ,  William Whitford ,  Richard Hassett ,  William Biddle ,  Jeffrey Biddle ,  Laurel Biddle ,  Christine Biddle ,  Robert Radominski ,  Barbara Dadey

发明人： Richard Fike ,  William Whitford ,  Richard Hassett ,  William Biddle ,  Jeffrey Biddle ,  Laurel Biddle ,  Christine Biddle ,  Robert Radominski ,  Barbara Dadey

IPC分类号： B01J8/18 ,  C12N5/00

CPC分类号： C12N5/0018 ,  C12N1/00 ,  C12N2500/36 ,  C12N2500/60

摘要： The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.

公开(公告)号：US20060003448A1

公开(公告)日：2006-01-05

申请号：US11024053

申请日：2004-12-29

申请人： Richard Fike ,  William Whitford ,  William Biddle ,  Laurel Biddle ,  Christine Biddle ,  Jeffrey Biddle ,  Richard Hassett ,  Barbara Dadey

发明人： Richard Fike ,  William Whitford ,  William Biddle ,  Laurel Biddle ,  Christine Biddle ,  Jeffrey Biddle ,  Richard Hassett ,  Barbara Dadey

IPC分类号： C12N5/02

CPC分类号： C12N5/0018

摘要： The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention is also directed to methods of production of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.

公开(公告)号：US20060003447A1

公开(公告)日：2006-01-05

申请号：US11024051

申请日：2004-12-29

申请人： Richard Fike ,  William Whitford ,  William Biddle ,  Laurel Biddle ,  Christine Biddle ,  Jeffrey Biddle

发明人： Richard Fike ,  William Whitford ,  William Biddle ,  Laurel Biddle ,  Christine Biddle ,  Jeffrey Biddle

IPC分类号： C12N5/02

CPC分类号： C12N5/0018 ,  C12N2500/60

摘要： The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention is also directed to methods of production of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.

公开(公告)号：US20080261308A1

公开(公告)日：2008-10-23

申请号：US12018035

申请日：2008-01-22

申请人： Richard M. Fike ,  William Whitford ,  Richard F. Hassett ,  Jeffrey W. Biddle ,  Laurel Biddle ,  Christine M. Biddle ,  Robert C. Radominski ,  Barbara M. Dadey

发明人： Richard M. Fike ,  William Whitford ,  Richard F. Hassett ,  Jeffrey W. Biddle ,  Laurel Biddle ,  Christine M. Biddle ,  Robert C. Radominski ,  Barbara M. Dadey

IPC分类号： C12N5/00

CPC分类号： C12N5/0018 ,  C12N1/00 ,  C12N2500/36 ,  C12N2500/60

摘要： The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.

摘要翻译： 本发明涉及营养培养基，培养基，培养基和缓冲剂制剂。 本发明提供粉末营养培养基，培养基和中等亚组分制剂，例如细胞培养基补充剂（包括粉末状血清如粉状胎牛血清（FBS）），培养基亚组制剂和包含所有必需营养因子的细胞培养基 促进细胞的体外培养。 本发明进一步提供粉末缓冲剂制剂，其在用溶剂重构时产生特定的离子和pH条件。 本发明提供了制备培养基，培养基，培养基和缓冲液制剂的方法，还提供用于培养原核和真核细胞，特别是细菌细胞，酵母细胞，植物细胞和动物细胞（包括人细胞）的试剂盒和方法，其使用 这些干粉营养介质，培养基补充剂，培养基和缓冲液配方。

公开(公告)号：US20130109094A1

公开(公告)日：2013-05-02

申请号：US13614588

申请日：2012-09-13

申请人： Richard M. Fike ,  William Whitford ,  Richard F. Hassett ,  Jeffrey W. Biddle ,  Laurel Biddle ,  Christine M. Biddle ,  Robert C. Radominski ,  Barbara M. Dadey

发明人： Richard M. Fike ,  William Whitford ,  Richard F. Hassett ,  Jeffrey W. Biddle ,  Laurel Biddle ,  Christine M. Biddle ,  Robert C. Radominski ,  Barbara M. Dadey

IPC分类号： C12N5/00

CPC分类号： C12N5/0018 ,  C12N1/00 ,  C12N2500/36 ,  C12N2500/60

摘要： The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.

摘要翻译： 本发明涉及营养培养基，培养基，培养基和缓冲剂制剂。 本发明提供粉末营养培养基，培养基和中等亚组分制剂，例如细胞培养基补充剂（包括粉末状血清如粉状胎牛血清（FBS）），培养基亚组制剂和包含所有必需营养因子的细胞培养基 促进细胞的体外培养。 本发明进一步提供粉末缓冲剂制剂，其在用溶剂重构时产生特定的离子和pH条件。 本发明提供了制备培养基，培养基，培养基和缓冲液制剂的方法，还提供用于培养原核和真核细胞，特别是细菌细胞，酵母细胞，植物细胞和动物细胞（包括人细胞）的试剂盒和方法，其使用 这些干粉营养介质，培养基补充剂，培养基和缓冲液配方。

公开(公告)号：US20080311660A1

公开(公告)日：2008-12-18

申请号：US11969338

申请日：2008-01-04

申请人： Richard M. Fike ,  William Whitford ,  Richard F. Hassett ,  Jeffrey W. Biddle ,  Laurel Biddle ,  Christine M. Biddle ,  Robert C. Radominski ,  Barbara M. Dadey

发明人： Richard M. Fike ,  William Whitford ,  Richard F. Hassett ,  Jeffrey W. Biddle ,  Laurel Biddle ,  Christine M. Biddle ,  Robert C. Radominski ,  Barbara M. Dadey

IPC分类号： C12N5/06

CPC分类号： C12N5/0018 ,  C12N1/00 ,  C12N2500/36 ,  C12N2500/60

摘要： The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.

摘要翻译： 本发明涉及营养培养基，培养基，培养基和缓冲剂制剂。 本发明提供粉末营养培养基，培养基和中等亚组分制剂，例如细胞培养基补充剂（包括粉末状血清如粉状胎牛血清（FBS）），培养基亚组制剂和包含所有必需营养因子的细胞培养基 促进细胞的体外培养。 本发明进一步提供粉末缓冲剂制剂，其在用溶剂重构时产生特定的离子和pH条件。 本发明提供了制备培养基，培养基，培养基和缓冲液制剂的方法，还提供用于培养原核和真核细胞，特别是细菌细胞，酵母细胞，植物细胞和动物细胞（包括人细胞）的试剂盒和方法，其使用 这些干粉营养介质，培养基补充剂，培养基和缓冲液配方。