公开(公告)号：US5046392A

公开(公告)日：1991-09-10

申请号：US334302

申请日：1989-04-06

申请人： Richard Keon ,  Luther Morris

发明人： Richard Keon ,  Luther Morris

IPC分类号： B26D1/18 ,  C03B37/16

CPC分类号： C03B37/16 ,  B26D1/18 ,  Y10T83/0207 ,  Y10T83/0304 ,  Y10T83/0341 ,  Y10T83/0348 ,  Y10T83/7507 ,  Y10T83/7763 ,  Y10T83/8822

摘要： A method and apparatus for severing a length of insulating batting and simultaneously cutting only the insulating material thereof is provided. The insulating material between the severed edge of the insulation batting and the cutting line through the insulating material can be removed to expose a length of backing which can be secured to the uncut portion of the insulation batting length when the insulation batting length is wrapped around a length of a heating and cooling duct or the like. The apparatus includes a pair of rotating cutting wheels rotatably mounted on a carriage movable along an elongate carriage rail. A pinion gear mounted to each rotating cutting wheel for rotation therewith meshes with a rack secured to the elongate carriage rail to cause rotation of the rotating cutting wheel in response to movement of the carriage along the carriage rail.

摘要翻译： 提供一种用于切断绝缘棉絮的长度并同时仅切割其绝缘材料的方法和装置。 绝缘棉絮的切断边缘与穿过绝缘材料的切割线之间的绝缘材料可以被去除以暴露一定长度的背衬，当将绝缘棉絮长度缠绕在绝缘棉絮长度上时，可以将其固定到绝缘bat击长度的未切割部分 加热和冷却管道的长度等。 该装置包括一对旋转的切割轮，其可旋转地安装在可沿着细长滑架导轨移动的滑架上。 安装到每个旋转切割轮上的小齿轮用于随其旋转啮合，其具有固定到细长滑架导轨上的齿条，以响应于滑架沿着滑架导轨移动而旋转的切割轮的旋转。

公开(公告)号：US20050039235A1

公开(公告)日：2005-02-17

申请号：US10869040

申请日：2004-06-17

申请人： Maurice Moloney ,  Joseph Boothe ,  Richard Keon ,  Cory Nykiforuk ,  Gijs Van Rooijen

发明人： Maurice Moloney ,  Joseph Boothe ,  Richard Keon ,  Cory Nykiforuk ,  Gijs Van Rooijen

IPC分类号： A01H1/00 ,  C07K14/62 ,  C12N15/82

CPC分类号： C07K14/62 ,  C12N15/8257

摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5′ to 3′ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.

摘要翻译： 描述了在植物中产生胰岛素的方法。 在一个实施方案中，本发明提供了一种用于在植物中表达胰岛素的方法，包括：（a）提供嵌合核酸构建体，其以5'至3'的转录方向包含可操作地连接的组分：（i）核酸 能够控制植物种子细胞中表达的序列; 和（ii）编码胰岛素多肽的核酸序列; （b）将嵌合核酸构建体引入植物细胞; 和（c）将植物细胞生长成能种植种子的成熟植物，其中种子表达胰岛素。

公开(公告)号：US07547821B2

公开(公告)日：2009-06-16

申请号：US10869040

申请日：2004-06-17

申请人： Maurice M. Moloney ,  Joseph Boothe ,  Richard Keon ,  Cory Nykiforuk ,  Gijs Van Rooijen

发明人： Maurice M. Moloney ,  Joseph Boothe ,  Richard Keon ,  Cory Nykiforuk ,  Gijs Van Rooijen

IPC分类号： C12N15/82 ,  A01H5/00 ,  C07H21/04

CPC分类号： C07K14/62 ,  C12N15/8257

摘要： Commercial production of human insulin can be affected via transgenic expression in plant seeds. Thus, levels of insulin accumulation exceeding 0.1% of total cellular protein can be achieved recombinantly, through expression of the insulin with a single-chain antibody as a fusion partner. Production in seeds offers flexibility in storage and shipment of insulin as a raw material, and insulin retains its activity upon extraction from stored seed. Further, the amount of biomass subjected to extraction is limited, due to the relatively low water content of plant seeds.

摘要翻译： 人类胰岛素的商业生产可以通过植物种子中的转基因表达来影响。 因此，通过以单链抗体作为融合配偶体的胰岛素的表达，可以重组地实现超过总细胞蛋白质的0.1％的胰岛素积聚的水平。 种子生产提供了作为原料的胰岛素储存和运输的灵活性，胰岛素从储存的种子提取时保留其活性。 此外，由于植物种子的含水量相对较低，所以提取的生物质的量受到限制。