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公开(公告)号：US07910362B2
公开(公告)日：2011-03-22
申请号：US11995398
申请日：2006-07-12
申请人： Rika Ueda , Kazutomi Kusano , Yasushi Yamazoe , Kiyoshi Nagata
发明人： Rika Ueda , Kazutomi Kusano , Yasushi Yamazoe , Kiyoshi Nagata
CPC分类号： C12N15/1086
摘要： A reporter vector which can evaluate the ability of a drug to induce CYP1A2 or both of CYP1A1 and CYP1A2 and a method for evaluation of the ability of a drug to induce CYP1A2 or both of CYP1A1 and CYP1A2 by using the reporter vector. A reporter system which can evaluate the ability of a drug capable of inducing CYP1A2 or both of CYP1A1 and CYP1A2 is completed by constructing a reporter vector having a reporter gene linked to the 3′ end of a region between CYP1A1 and CYP1A2 or a reporter vector having different reporter genes linked to the both ends of the region, respectively, so as to sandwich the region, and a reporter vector having a deletion mutation in the region, and confirming that the expression of a reporter molecule is increased by the drug capable of inducing CYP1A2 or both of CYP1A1 and CYP1A2 in the reporter system using the reporter vector.
摘要翻译： 可以评估药物诱导CYP1A2或CYP1A1和CYP1A2两者的能力的报告载体，以及通过使用报道载体评价药物诱导CYP1A2或CYP1A1和CYP1A2两者能力的方法。 可以通过构建具有与CYP1A1和CYP1A2之间的区域的3'末端连接的报道基因的报道载体或具有CYP1A1和CYP1A2两者的报道载体的报道载体来完成能够评价能够诱导CYP1A2或CYP1A1和CYP1A2两者的药物的能力的报道体系， 分别与区域两端连接的不同的报道基因以夹持该区域，以及在该区域具有缺失突变的报道载体，并证实报告分子的表达由能诱导的药物增加 CYP1A2或两者CYP1A1和CYP1A2在报告系统中使用报告载体。
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公开(公告)号：US20090317800A1
公开(公告)日：2009-12-24
申请号：US11995398
申请日：2006-07-12
申请人： Rika Ueda , Kazutomi Kusano , Yasushi Yamazoe , Kiyoshi Nagata
发明人： Rika Ueda , Kazutomi Kusano , Yasushi Yamazoe , Kiyoshi Nagata
CPC分类号： C12N15/1086
摘要： A reporter vector which can evaluate the ability of a drug to induce CYP1A2 or both of CYP1A1 and CYP1A2 and a method for evaluation of the ability of a drug to induce CYP1A2 or both of CYP1A1 and CYP1A2 by using the reporter vector. A reporter system which can evaluate the ability of a drug capable of inducing CYP1A2 or both of CYP1A1 and CYP1A2 is completed by constructing a reporter vector having a reporter gene linked to the 3′ end of a region between CYP1A1 and CYP1A2 or a reporter vector having different reporter genes linked to the both ends of the region, respectively, so as to sandwich the region, and a reporter vector having a deletion mutation in the region, and confirming that the expression of a reporter molecule is increased by the drug capable of inducing CYP1A2 or both of CYP1A1 and CYP1A2 in the reporter system using the reporter vector.
摘要翻译： 可以评估药物诱导CYP1A2或CYP1A1和CYP1A2两者的能力的报告载体，以及通过使用报道载体评价药物诱导CYP1A2或CYP1A1和CYP1A2两者能力的方法。 可以通过构建具有与CYP1A1和CYP1A2之间的区域的3'末端连接的报道基因的报道载体或具有CYP1A1和CYP1A2两者的报道载体的报道载体来完成能够评价能够诱导CYP1A2或CYP1A1和CYP1A2两者的药物的能力的报道体系， 分别与区域两端连接的不同的报道基因以夹持该区域，以及在该区域具有缺失突变的报道载体，并证实报告分子的表达由能诱导的药物增加 CYP1A2或两者CYP1A1和CYP1A2在报告系统中使用报告载体。
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