公开(公告)号：US20070249820A1

公开(公告)日：2007-10-25

申请号：US11428779

申请日：2006-07-05

申请人： Robert Batey ,  Jeffrey Kieft

发明人： Robert Batey ,  Jeffrey Kieft

IPC分类号： C07H21/04

CPC分类号： C12N15/111 ,  C07H21/04 ,  C12N2310/121 ,  C12N2310/123 ,  C12N2310/127 ,  C12N2310/351 ,  C12N2310/3519 ,  C12N2320/10

摘要： Activatable ribozymal purification constructs are used to rapidly and efficiently purify RNA, including non-denatured RNA. The activatable ribozymal purification construct includes an activatable ribozyme covalently bound to a target RNA moiety. The activatable ribozyme is attached to an immobilizing moiety, which is capable of binding to solid support. Upon activation, the ribozyme cleaves the target RNA moiety thereby producing purified target RNA.

摘要翻译： 可活化的核酶纯化构建体用于快速和有效地纯化RNA，包括非变性RNA。 可激活的核酶纯化构建体包含与靶RNA部分共价结合的可激活核酶。 可活化的核酶连接到能够结合固体支持物的固定部分。 激活后，核酶切割靶RNA部分，从而产生纯化的靶RNA。

公开(公告)号：US08084399B2

公开(公告)日：2011-12-27

申请号：US12540246

申请日：2009-08-12

申请人： Jaehoon Yu ,  Jeffrey Kieft ,  Su Jin Lee

发明人： Jaehoon Yu ,  Jeffrey Kieft ,  Su Jin Lee

IPC分类号： C40B30/04 ,  A61K38/00

CPC分类号： C07K7/08

摘要： The present invention relates to a screening method of an amphiphilic peptide specifically binding to hairpin RNA, more precisely a screening method of an amphiphilic peptide having specificity and strong binding strength to target hairpin RNA using peptide library comprising those peptides having modifications of both hydrophilic face and hydrophobic face. The method of the present invention provides a screening method of an amphiphilic peptide which is specific to hairpin RNA. So, the peptide selected by the method of the present invention can be effectively used for the study of hairpin RNA functions and for the production of a novel drug using an artificial peptide binding to a hairpin RNA target.

摘要翻译： 本发明涉及特异性结合发夹RNA的两亲性肽的筛选方法，更确切地说，使用肽文库的具有特异性和对靶发夹RNA的强结合强度的两亲肽的筛选方法，所述肽文库包括具有亲水性面部和 疏水面。 本发明的方法提供了对发夹RNA特异性的两亲肽的筛选方法。 因此，通过本发明的方法选择的肽可以有效地用于研究发夹RNA功能，并且可以使用结合发夹RNA靶的人工肽来生产新药。

公开(公告)号：US20100173796A1

公开(公告)日：2010-07-08

申请号：US12540246

申请日：2009-08-12

申请人： Jaehoon YU ,  Jeffrey Kieft ,  Su Jin Lee

发明人： Jaehoon YU ,  Jeffrey Kieft ,  Su Jin Lee

IPC分类号： C40B30/04 ,  C40B40/10

CPC分类号： C07K7/08

摘要： The present invention relates to a screening method of an amphiphilic peptide specifically binding to hairpin RNA, more precisely a screening method of an amphiphilic peptide having specificity and strong binding strength to target hairpin RNA using peptide library comprising those peptides having modifications of both hydrophilic face and hydrophobic face. The method of the present invention provides a screening method of an amphiphilic peptide which is specific to hairpin RNA. So, the peptide selected by the method of the present invention can be effectively used for the study of hairpin RNA functions and for the production of a novel drug using an artificial peptide binding to a hairpin RNA target.

摘要翻译： 本发明涉及特异性结合发夹RNA的两亲性肽的筛选方法，更确切地说，使用肽文库的具有特异性和对靶发夹RNA的强结合强度的两亲肽的筛选方法，所述肽文库包括具有亲水性面部和 疏水面。 本发明的方法提供了对发夹RNA特异性的两亲肽的筛选方法。 因此，通过本发明的方法选择的肽可以有效地用于研究发夹RNA功能，并且可以使用结合发夹RNA靶的人工肽来生产新药。