公开(公告)号：US20060172278A1

公开(公告)日：2006-08-03

申请号：US11202848

申请日：2005-08-12

申请人： Robert Bonner ,  Thomas Pohida ,  Michael Emmert-Buck ,  Michael Tangrea ,  Rodrigo Chuaqui

发明人： Robert Bonner ,  Thomas Pohida ,  Michael Emmert-Buck ,  Michael Tangrea ,  Rodrigo Chuaqui

IPC分类号： C12Q1/00 ,  G01N1/30 ,  C12M1/34

CPC分类号： G01N33/543 ,  G01N33/56966 ,  G01N2001/284 ,  Y10T428/2813

摘要： A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.

公开(公告)号：US20060134692A1

公开(公告)日：2006-06-22

申请号：US10543218

申请日：2003-07-23

申请人： Michael Emmert-Buck ,  Michael Tangrea ,  Robert Bonner ,  Rodrigo Chuaqui

发明人： Michael Emmert-Buck ,  Michael Tangrea ,  Robert Bonner ,  Rodrigo Chuaqui

IPC分类号： G01N33/53 ,  G01N33/537 ,  G01N33/543

CPC分类号： G01N33/56966 ,  G01N33/543 ,  Y10T428/2813 ,  Y10T428/2839

摘要： A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target). The immunoreagent can alter the transfer surface directly (for example with a heat generating enzyme carried by the immunoreagent), or indirectly (for example by changing a characteristic of the target). In some embodiments, the immunoreagent deposits a precipitate in the target that increases its light absorption relative to surrounding tissue, such that the biological specimen can be exposed to light to selectively heat the target. Alternatively, the immunoreagent is an immunofluorescent agent that carries a fluorophore that absorbs light and emits heat.

公开(公告)号：US20070126252A1

公开(公告)日：2007-06-07

申请号：US11164734

申请日：2005-12-02

申请人： Leo Castagno ,  Melvin Price ,  James Spillman ,  Bradley Price ,  Robert Bonner

发明人： Leo Castagno ,  Melvin Price ,  James Spillman ,  Bradley Price ,  Robert Bonner

IPC分类号： B60P3/00

CPC分类号： B60P3/34

摘要： An interior space expansion system for use with a structure needing collapsible and/or expandable space, including: a base structure having an aperture therethrough; a movable structure disposed within a central region of the base structure when in a sunken position and substantially exterior the base structure when in an elevated position; and a lifting system coupled to the base structure and the movable structure. The base structure is a trailer or recreational vehicle. The movable structure includes a cab a carriage and a footing. The lifting system couples to the footing and transitions the movable structure between the sunken and elevated positions. The lifting system is not substantially disposed within the central region of the base structure when the movable structure is in the elevated position. There is a cap member for covering the aperture when the movable structure is in the sunken position.

摘要翻译： 一种用于需要可折叠和/或可膨胀空间的结构的内部空间膨胀系统，包括：具有穿过其中的孔的基座结构; 可移动结构，当处于提升位置时，当处于沉没位置并且基本上在基部外部时，设置在基部结构的中心区域内; 以及联接到基部结构和可移动结构的提升系统。 基座结构是拖车或休闲车。 可移动结构包括驾驶室和托架。 提升系统耦合到基脚，并且可移动结构在凹陷和升高位置之间转移。 当可移动结构处于升高位置时，提升系统基本上不设置在基座结构的中心区域内。 当可移动结构处于沉没位置时，存在用于覆盖孔的盖构件。