公开(公告)号：US09598473B2

公开(公告)日：2017-03-21

申请号：US14124629

申请日：2012-06-08

申请人： Robert Cornelis Schuurink ,  Michael Albertus Haring ,  Eleni Spyropoulou

发明人： Robert Cornelis Schuurink ,  Michael Albertus Haring ,  Eleni Spyropoulou

IPC分类号： C07K14/415 ,  A01H5/12 ,  A01H5/00 ,  C12N15/82 ,  A01H1/02

CPC分类号： C12N15/8243 ,  A01H1/02 ,  A01H1/04 ,  A01H5/00 ,  A01H5/12 ,  C07K14/415 ,  C12N15/8286 ,  C12P5/007 ,  Y02A40/162

摘要： The present invention relates to identification and isolation of zinc finger transcription factor in tomato that specifically expresses in glandular trichomes of Solanum lycopersicum cultivar Moneymaker and binds to the promoters of the genes encoding Terpene Synthase 5 (also known as Monoterpene Synthase 1) and Terpene Synthase 11 (also known as Sesquiterpene Synthase 1). The invention provides the isolated, recombinant or synthetic polynucleotides encoding the polypeptide sequences of SEQ ID NO:2 and variants and fragments thereof. The invention also provides constructs, vectors, host cells and plants genetically modified to contain the polynucleotides of the invention. The methods for producing plants with altered levels of terpenes, including transformed and mutant plants, are also provided.

公开(公告)号：US20140173771A1

公开(公告)日：2014-06-19

申请号：US14124629

申请日：2012-06-08

申请人： Robert Cornelis Schuurink ,  Michael Albertus Haring ,  Eleni Spyropoulou

发明人： Robert Cornelis Schuurink ,  Michael Albertus Haring ,  Eleni Spyropoulou

IPC分类号： C07K14/415 ,  A01H5/12 ,  A01H5/00 ,  C12N15/82 ,  A01H1/02

CPC分类号： C12N15/8243 ,  A01H1/02 ,  A01H1/04 ,  A01H5/00 ,  A01H5/12 ,  C07K14/415 ,  C12N15/8286 ,  C12P5/007 ,  Y02A40/162

摘要： The present invention relates to identification and isolation of zinc finger transcription factor in tomato that specifically expresses in glandular trichomes of Solanum lycopersicum cultivar Moneymaker and binds to the promoters of the genes encoding Terpene Synthase 5 (also known as Monoterpene Synthase 1) and Terpene Synthase 11 (also known as Sesquiterpene Synthase 1). The invention provides the isolated, recombinant or synthetic polynucleotides encoding the polypeptide sequences of SEQ ID NO:2 and variants and fragments thereof. The invention also provides constructs, vectors, host cells and plants genetically modified to contain the polynucleotides of the invention. The methods for producing plants with altered levels of terpenes, including transformed and mutant plants, are also provided.

摘要翻译： 本发明涉及番茄中锌指转录因子的鉴定和分离，其特异性表达在番茄茄子栽培品种Moneymaker的腺体毛中，并结合编码萜烯合酶5（也称为单萜合酶1）和萜烯合酶11的基因的启动子 （也称为倍半萜合酶1）。 本发明提供编码SEQ ID NO：2的多肽序列的分离的，重组的或合成的多核苷酸及其变体和片段。 本发明还提供构建体，载体，宿主细胞和遗传修饰以包含本发明的多核苷酸的植物。 还提供了用于生产具有改变的萜烯含量的植物的方法，包括转化和突变植物。