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公开(公告)号：US09598473B2
公开(公告)日：2017-03-21
申请号：US14124629
申请日：2012-06-08
IPC分类号： C07K14/415 , A01H5/12 , A01H5/00 , C12N15/82 , A01H1/02
CPC分类号： C12N15/8243 , A01H1/02 , A01H1/04 , A01H5/00 , A01H5/12 , C07K14/415 , C12N15/8286 , C12P5/007 , Y02A40/162
摘要： The present invention relates to identification and isolation of zinc finger transcription factor in tomato that specifically expresses in glandular trichomes of Solanum lycopersicum cultivar Moneymaker and binds to the promoters of the genes encoding Terpene Synthase 5 (also known as Monoterpene Synthase 1) and Terpene Synthase 11 (also known as Sesquiterpene Synthase 1). The invention provides the isolated, recombinant or synthetic polynucleotides encoding the polypeptide sequences of SEQ ID NO:2 and variants and fragments thereof. The invention also provides constructs, vectors, host cells and plants genetically modified to contain the polynucleotides of the invention. The methods for producing plants with altered levels of terpenes, including transformed and mutant plants, are also provided.
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公开(公告)号：US20140173771A1
公开(公告)日：2014-06-19
申请号：US14124629
申请日：2012-06-08
IPC分类号： C07K14/415 , A01H5/12 , A01H5/00 , C12N15/82 , A01H1/02
CPC分类号： C12N15/8243 , A01H1/02 , A01H1/04 , A01H5/00 , A01H5/12 , C07K14/415 , C12N15/8286 , C12P5/007 , Y02A40/162
摘要： The present invention relates to identification and isolation of zinc finger transcription factor in tomato that specifically expresses in glandular trichomes of Solanum lycopersicum cultivar Moneymaker and binds to the promoters of the genes encoding Terpene Synthase 5 (also known as Monoterpene Synthase 1) and Terpene Synthase 11 (also known as Sesquiterpene Synthase 1). The invention provides the isolated, recombinant or synthetic polynucleotides encoding the polypeptide sequences of SEQ ID NO:2 and variants and fragments thereof. The invention also provides constructs, vectors, host cells and plants genetically modified to contain the polynucleotides of the invention. The methods for producing plants with altered levels of terpenes, including transformed and mutant plants, are also provided.
摘要翻译： 本发明涉及番茄中锌指转录因子的鉴定和分离，其特异性表达在番茄茄子栽培品种Moneymaker的腺体毛中，并结合编码萜烯合酶5（也称为单萜合酶1）和萜烯合酶11的基因的启动子 （也称为倍半萜合酶1）。 本发明提供编码SEQ ID NO：2的多肽序列的分离的，重组的或合成的多核苷酸及其变体和片段。 本发明还提供构建体，载体，宿主细胞和遗传修饰以包含本发明的多核苷酸的植物。 还提供了用于生产具有改变的萜烯含量的植物的方法，包括转化和突变植物。
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公开(公告)号：US09234193B2
公开(公告)日：2016-01-12
申请号：US14122579
申请日：2012-05-31
CPC分类号： C12N15/8286 , A01H1/04 , C12N9/88 , C12N15/8239 , C12N15/8243 , C12P5/007 , C12Y402/00 , C12Y402/03053 , C12Y402/03054 , C12Y402/03082 , Y02A40/162
摘要： An isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising the nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves, are provided. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.
摘要翻译： 本发明提供了分离的编码7-表位安全壳合成酶的核酸分子，包含核酸分子的嵌合基因，包含该核酸分子的载体以及分离的7-表质抗原合成酶本身。 此外，提供了转基因植物和植物细胞，其包含编码7-表位活性物质合成酶的基因，任选地整合在其基因组中，以及用于制备这种植物和细胞的方法。 提供具有增强的害虫抗性的茄科植物和植物部分（种子，水果，叶子等）。
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公开(公告)号：US08674175B2
公开(公告)日：2014-03-18
申请号：US13132702
申请日：2009-12-02
CPC分类号： C12N9/88 , C12N15/8243 , C12P5/007
摘要： A new farnesene synthase was isolated from tomato. The farnesene synthase shows surprising properties with regard to the end products formed and its gene has, on a nucleotide level, low sequence identity with known farnesene synthase genes from other sources. The invention relates to isolated polynucleotides, polypeptides encoded by said polynucleotides, genetic constructs, vectors, hosts, in particular plants, harboring such polynucleotides, polypeptides and genetic constructs, and seed derived from such plants.
摘要翻译： 从番茄中分离出新的法呢烯合成酶。 法呢烯合酶对于形成的最终产物显示出惊人的性质，其基因在核苷酸水平上具有与其他来源的已知法呢烯合酶基因的低序列同一性。 本发明涉及分离的多核苷酸，由所述多核苷酸编码的多肽，遗传构建体，载体，宿主，特别是植物，携带这种多核苷酸，多肽和遗传构建体，以及衍生自该植物的种子。
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公开(公告)号：US20110113512A1
公开(公告)日：2011-05-12
申请号：US12809695
申请日：2008-12-16
申请人： Petronella Martina Bleeker , Christianus Cornelis Nicolaas Van Schie , Paul Johan Diergaarde , Robert Cornelis Schuurink , Michael Albertus Haring , Michiel Teodoor Jan De Both
发明人： Petronella Martina Bleeker , Christianus Cornelis Nicolaas Van Schie , Paul Johan Diergaarde , Robert Cornelis Schuurink , Michael Albertus Haring , Michiel Teodoor Jan De Both
CPC分类号： C12N15/8223
摘要： Trichome specific plant promoters are provided herein. Also provided are transgenic cells and organisms, especially plant cell and plants, comprising an trichome specific promoter and methods for expressing nucleic acid sequences in cells and organisms using trichome specific promoters.
摘要翻译： 本文提供了Trichome特异性植物启动子。 还提供了转基因细胞和生物体，特别是包含毛状体特异性启动子的植物细胞和植物，以及使用毛发特异性启动子在细胞和生物体中表达核酸序列的方法。
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公开(公告)号：US20110307972A1
公开(公告)日：2011-12-15
申请号：US13132702
申请日：2009-12-02
IPC分类号： C12N15/82 , C12N1/21 , C12N1/19 , C12N5/10 , A01H5/10 , C12P5/02 , C12Q1/68 , C07H21/04 , A01H5/00 , C12N15/63 , C12N9/88
CPC分类号： C12N9/88 , C12N15/8243 , C12P5/007
摘要： A new farnesene synthase was isolated from tomato. The farnesene synthase shows surprising properties with regard to the end products formed and its gene has, on a nucleotide level, low sequence identity with known farnesene synthase genes from other sources. The invention relates to isolated polynucleotides, polypeptides encoded by said polynucleotides, genetic constructs, vectors, hosts, in particular plants, harbouring such polynucleotides, polypeptides and genetic constructs, and seed derived from such plants.
摘要翻译： 从番茄中分离出新的法呢烯合成酶。 法呢烯合酶对于形成的最终产物显示出惊人的性质，其基因在核苷酸水平上具有与其他来源的已知法呢烯合酶基因的低序列同一性。 本发明涉及分离的多核苷酸，由所述多核苷酸编码的多肽，遗传构建体，载体，宿主，特别是植物，携带这种多核苷酸，多肽和遗传构建体，以及衍生自该植物的种子。
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