公开(公告)号：US20080235828A1

公开(公告)日：2008-09-25

申请号：US11933234

申请日：2007-10-31

申请人： Robert D. HILL ,  Fawzi A. Razam ,  Ashraf El-Kereamy ,  Santosh Kumar

发明人： Robert D. HILL ,  Fawzi A. Razam ,  Ashraf El-Kereamy ,  Santosh Kumar

IPC分类号： C12N15/82 ,  C12N15/11 ,  C12N5/04 ,  A01H5/00

CPC分类号： C07K14/415

摘要： Compositions and methods for modifying the sensitivity to abscisic acid, of seeds and vegetative tissues of plants. The compositions comprise novel nucleotide molecules configured for expressing an abscisic acid-binding protein, i.e., ABAP1, comprising an amino acid sequence set forth in SEQ ID NO: 10. The methods comprise the steps of constructing a nucleotide molecule by fusing a nucleotide coding sequence set forth in SEQ ID NO: 9 with a regulatory nucleotide sequence selected for over-expression or for under-expression of the coding sequence, stably introducing the nucleotide molecule into the genome of a recipient cell from a seed-producing plant, culturing the recipient cell into a whole recipient plant and then further culturing the recipient plant to produce mature recipient seeds, and harvesting the recipient seeds. The recipient seeds and whole plants produced therefrom have modified sensitivities to ABA thereby enabling regulation of germination, physiological function and plant productivity.

摘要翻译： 用于改变对植物的脱落酸，种子和营养组织的敏感性的组合物和方法。 所述组合物包含配置用于表达包含SEQ ID NO：10所示氨基酸序列的脱落酸结合蛋白即ABAP1的新型核苷酸分子。所述方法包括以下步骤：通过将核苷酸编码序列 如SEQ ID NO：9所示，具有选择用于编码序列的过表达或低表达的调节核苷酸序列，从种子产生植物稳定地将核苷酸分子引入受体细胞的基因组，培养受体 细胞进入整个受体植物，然后进一步培养受体植物以产生成熟的受精种子，并收获受体种子。 由此产生的受体种子和整株植物对ABA具有修饰敏感性，从而能够调节发芽，生理功能和植物生产力。