公开(公告)号：US06569685B1

公开(公告)日：2003-05-27

申请号：US09412732

申请日：1999-10-05

申请人： Robert H. Carlson ,  Ian E. Burbulis ,  Roger Brent

发明人： Robert H. Carlson ,  Ian E. Burbulis ,  Roger Brent

IPC分类号： G01N2162

CPC分类号： G01N33/582 ,  G01N33/542 ,  G01N33/6803

摘要： A system and method for characterizing protein molecules. A protein molecule of interest is isolated from other types of protein molecules. The protein molecule of interest is modified to a one-dimensional structure from the natural three-dimensional structure of the protein molecule. Each of a first type of amino acid residue of the protein molecule is labeled with a first tag. Each of a second type of amino acid residue of the protein molecule is labeled with a second tag. The first and second tags impart to the protein molecule a detectable set of characteristic ancillary properties that facilitates distinction of the protein molecule of interest from other types of protein molecules. When these ancillary properties are detected, a fingerprint of the protein molecule is revealed. A listing of known protein molecules and of the fingerprints corresponding to each of the known protein molecules serves as a library to facilitate identification of unknown proteins. A fingerprint of a protein molecule of interest determined in the same manner as the fingerprints of known proteins listed in the library is compared with fingerprints of the known protein molecules to identify the protein molecule of interest.

摘要翻译： 用于表征蛋白质分子的系统和方法。 感兴趣的蛋白质分子从其他类型的蛋白质分子中分离出来。 将目的蛋白质分子从蛋白质分子的天然三维结构修饰成一维结构。 蛋白质分子的第一类氨基酸残基中的每一个用第一标签标记。 蛋白质分子的第二类型的氨基酸残基中的每一个用第二个标签标记。 第一和第二标签赋予蛋白质分子可检测的一组特征性辅助性质，其有助于将目的蛋白质分子与其它类型的蛋白质分子区分开。 当检测到这些辅助性质时，显示出蛋白质分子的指纹图谱。 已知蛋白质分子和对应于每个已知蛋白质分子的指纹的列表用作有助于鉴定未知蛋白质的文库。 将以与文库中列出的已知蛋白质的指纹相同的方式测定的感兴趣的蛋白质分子的指纹与已知蛋白质分子的指纹进行比较，以鉴定感兴趣的蛋白质分子。