公开(公告)号：US06867350B2

公开(公告)日：2005-03-15

申请号：US09859822

申请日：2001-05-17

申请人： Robert J. Ferl ,  Paul C. Sehnke ,  Hwa Jee Chung ,  Ke Wu ,  L. Curtis Hannah

发明人： Robert J. Ferl ,  Paul C. Sehnke ,  Hwa Jee Chung ,  Ke Wu ,  L. Curtis Hannah

IPC分类号： C12N15/29 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8245

摘要： The subject invention concerns materials and methods for enhancing starch production in plants. Starch production is enhanced, relative to levels observed in wildtype or control plants, by reduction of the plant 14-3-3 protein(s) which subsequently results in increased accumulation of starch in the plant. In one embodiment, the 14-3-3 protein expression is reduced using polynucleotides that are antisense to the 14-3-3 gene sequences expressed in the plant. In another embodiment, the 14-3-3 protein expression is reduced by “knockout” of a 14-3-3 gene or gene sequences. The subject invention also pertains to transformed and transgenic plants that have polynucleotides that are antisense to the 14-3-3 gene sequences expressed in the plant, wherein the transformed and transgenic plants exhibit enhanced starch production. The subject invention also pertains to “knockout” plants in which the normal functional 14-3-3 gene in the plant is deleted or replaced with a non-functional form of the gene. The subject invention also concerns the “antisense” polynucleotides of the invention that when introduced into a plant cell can function to effectively reduce expression of the 14-3-3 proteins in a plant.