公开(公告)号：US4781464A

公开(公告)日：1988-11-01

申请号：US851233

申请日：1986-04-14

申请人： Robert W. Allington ,  Wylee D. Brunken ,  Lawrence L. Sedlak

发明人： Robert W. Allington ,  Wylee D. Brunken ,  Lawrence L. Sedlak

IPC分类号： G01N21/59 ,  G01N27/447 ,  G01N21/86

CPC分类号： G01N27/44721 ,  G01N21/5911

摘要： To scan gels optically, a gel scanner includes a gel holder and an optical system mounted for motion with respect to each other. The optical system includes a tungsten halogen lamp, first aperture, second aperture, beam splitter and first and second light paths; the first aperture having a dimension in the direction of motion of the gel with respect to the optical system of less than 0.5 millimeter and a dimension in a direction transverse to the direction of motion of less than 10 millimeters. The lamp and first aperture are mounted on one side of said gel holder and the second aperture is mounted on the other side of said gel holder. The beam splitter transmits light in a first direction along the first light path and in a second direction along a second light path with the: (1) first light path including a first photodetector and an interference filter for passing light within the range of 400 nanometers wavelength to 700 nanometers wavelength to it; and (2) the second light path having a second photodetector.

摘要翻译： 为了光学扫描凝胶，凝胶扫描仪包括凝胶保持器和安装成相对于彼此运动的光学系统。 光学系统包括卤钨灯，第一光圈，第二光圈，光束分离器和第一和第二光路; 所述第一孔具有在所述凝胶相对于所述光学系统的运动方向上的尺寸小于0.5毫米，并且在垂直于所述运动方向的方向上的尺寸小于10毫米。 灯和第一孔安装在所述凝胶保持器的一侧上，第二孔安装在所述凝胶保持器的另一侧上。 分束器沿着第一光路沿着第一方向透射光，沿着第二光路在第二方向上透射光，其中：（1）第一光路包括第一光电检测器和用于使光在400纳米范围内通过的干涉滤光器 波长到700纳米波长; 和（2）第二光路具有第二光电检测器。