公开(公告)号：US20050148507A1

公开(公告)日：2005-07-07

申请号：US10833656

申请日：2004-04-28

申请人： Robert Wandl ,  Roman Necina ,  Henri Doods ,  Martin Lenter ,  Randolph Seidler

发明人： Robert Wandl ,  Roman Necina ,  Henri Doods ,  Martin Lenter ,  Randolph Seidler

IPC分类号： A61K38/17 ,  C07K14/475 ,  C07K14/52

CPC分类号： C07K14/523

摘要： The invention relates to a process for preparing pyroGlu-MCP-1 from recombinantly produced Gln-MCP-1, wherein Gln-MCP-1 is incubated at a temperature in the range from 30° C. and 80° C. in a buffer solution with a salt concentration in the range from 10 mM to 160 mM and a pH in the range from 2 to 7.5, until at least 90% of the MCP-1 is present in the form of the pyroGlu-MCP-1.

摘要翻译： 本发明涉及从重组产生的Gln-MCP-1制备pyroGlu-MCP-1的方法，其中Gln-MCP-1在30℃和80℃的温度下在缓冲溶液 盐浓度范围为10mM至160mM，pH范围为2至7.5，直至MCP-1的至少90％以pyroGlu-MCP-1的形式存在。