公开(公告)号：US20070087333A1

公开(公告)日：2007-04-19

申请号：US11506418

申请日：2006-08-18

申请人： Robertus Gruters ,  Carel van Baalen ,  Gustaaf Rimmelzwaan ,  Albertus Dominicus Marcellinus Osterhaus

发明人： Robertus Gruters ,  Carel van Baalen ,  Gustaaf Rimmelzwaan ,  Albertus Dominicus Marcellinus Osterhaus

IPC分类号： C12Q1/70 ,  C12Q1/68 ,  G01N33/567

CPC分类号： G01N33/5014 ,  C12Q1/6897 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5047

摘要： The invention relates to a novel non-radioactive method to detect cytolytic activity that provides a measure of the existence and magnitude of an immune response against a particular antigen or immunogen. Provided is a method for detecting cytolytic activity of cells or a substance against a population of target cells, comprising the steps of providing target cells with a first nucleic acid sequence encoding a reporter molecule and a second nucleic acid sequence encoding an antigen of interest; co-culturing the target cells with a sample containing cells or a substance suspected of having cytolytic activity; and detecting the viability of target cells provided with the reporter molecule. Also provided are a kit and a nucleic acid for use in a method according to the invention.

摘要翻译： 本发明涉及一种检测细胞溶解活性的新型非放射性方法，其提供针对特定抗原或免疫原的免疫应答的存在和量值的量度。 提供了一种用于检测细胞或靶向细胞群体的细胞溶解活性的方法，包括以下步骤：向靶细胞提供编码报道分子的第一核酸序列和编码感兴趣抗原的第二核酸序列; 用含有细胞或疑似具有细胞溶解活性的物质的样品共培养靶细胞; 并检测提供有报告分子的靶细胞的活力。 还提供了用于根据本发明的方法的试剂盒和核酸。