公开(公告)号：US20220057393A1

公开(公告)日：2022-02-24

申请号：US17482700

申请日：2021-09-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Barbara UPMEIER ,  Toralf ZARNT ,  Johannes POLZ

IPC: G01N33/543 ,  G01N33/68

Abstract: The present invention relates to a method for determining an analyte in a sample suspected to comprise said analyte, comprising a) contacting with said sample at least a first and a second capture compound for said analyte, wherein said first and second capture compounds are nonidentical capture compounds, and wherein said capture compounds compete in binding to said analyte; b) contacting said capture compounds contacted with said sample with a specifier, wherein said specifier competes in binding to said capture compounds with said analyte; c) determining the amount of complexes comprising said specifier and a capture compound; and d) determining said analyte in a sample based on the result of step c). The present invention further relates to a method for improving the specificity of an indirect immunoassay for determining an analyte, comprising replacing at least 10% of a capture compound by a non-identical capture compound; wherein the capture compound replaced competes in binding to said analyte with the capture compound introduced. The present invention further relates to kits, devices, and uses related to the aforementioned methods.