公开(公告)号：US20220317119A1

公开(公告)日：2022-10-06

申请号：US17838568

申请日：2022-06-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Toralf ZARNT ,  Dieter HEINDL ,  Alfons NICHTL ,  Frank SICHERL-BIRK

IPC: G01N33/543

Abstract: Disclosed is a method for measurement of an analyte in a microparticle-based analyte-specific binding assay, wherein the microparticles are coated with the first partner of a binding pair, the method involving mixing the coated microparticles, an analyte-specific binding agent conjugated to the second partner of the binding pair, and a sample suspected of containing or containing the analyte, wherein the second partner of the binding pair is bound to the analyte-specific binding agent via a linker having from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agent to the coated microparticles, separating the microparticles having the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.

公开(公告)号：US20220057393A1

公开(公告)日：2022-02-24

申请号：US17482700

申请日：2021-09-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Barbara UPMEIER ,  Toralf ZARNT ,  Johannes POLZ

IPC: G01N33/543 ,  G01N33/68

Abstract: The present invention relates to a method for determining an analyte in a sample suspected to comprise said analyte, comprising a) contacting with said sample at least a first and a second capture compound for said analyte, wherein said first and second capture compounds are nonidentical capture compounds, and wherein said capture compounds compete in binding to said analyte; b) contacting said capture compounds contacted with said sample with a specifier, wherein said specifier competes in binding to said capture compounds with said analyte; c) determining the amount of complexes comprising said specifier and a capture compound; and d) determining said analyte in a sample based on the result of step c). The present invention further relates to a method for improving the specificity of an indirect immunoassay for determining an analyte, comprising replacing at least 10% of a capture compound by a non-identical capture compound; wherein the capture compound replaced competes in binding to said analyte with the capture compound introduced. The present invention further relates to kits, devices, and uses related to the aforementioned methods.