公开(公告)号：US20230333123A1

公开(公告)日：2023-10-19

申请号：US18337018

申请日：2023-06-18

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Marius Fraefel ,  Stefanie Grimm ,  Thomas Hoffmann

IPC: G01N33/74 ,  G01N30/72

CPC classification number: G01N33/743 ,  G01N30/7233

Abstract: The present invention relates to a method for detecting and/or quantifying one or more steroids using mass spectrometry, said steroids comprising at least one 11-oxygenated steroids. The method of the invention comprises (i) extracting the one or more steroids from the sample using solid phase extraction (SPE) so as to obtain an SPE extract comprising the one or more steroids; (ii) concentrating the one or more steroids, said concentrating comprising evaporating solvent from the SPE-extract; and (iii) detecting or quantifying the one or more steroids in the sample using mass spectrometry.