公开(公告)号：US20170000396A9

公开(公告)日：2017-01-05

申请号：US13958404

申请日：2013-08-02

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Arnulf Staib ,  Reinhold Mischler ,  Martin Hajnsek ,  Harvey Buck ,  Walter Jernigan

IPC: A61B5/1486

CPC classification number: A61B5/14865 ,  A61B5/14532

Abstract: An in vivo amperometric sensor is provided for measuring the concentration of an analyte in a body fluid. The sensor comprises a counter electrode and a working electrode, and the working electrode comprises a sensing layer which is generally water permeable and arranged on a support member adjacent to a contact pad. The sensing layer comprises an immobilized enzyme capable of acting catalytically in the presence of the analyte to cause an electrical signal. The sensing layer has an upper surface facing the body fluid and a lower surface facing away from the body fluid, and the immobilized enzyme is distributed within the sensing layer in such a way that the enzyme concentration in the middle between the upper and lower surfaces is at least as high as on the upper surface of the sensing layer.

Abstract translation: 提供体内电流传感器用于测量体液中分析物的浓度。 传感器包括对电极和工作电极，并且工作电极包括通常为水可渗透并且布置在与接触垫相邻的支撑构件上的感测层。 感测层包含能够在分析物存在下催化作用以产生电信号的固定化酶。 感测层具有面向体液的上表面和背离体液的下表面，并且固定化酶分布在感测层内，使得上下表面中间的酶浓度为 至少与感测层的上表面一样高。

公开(公告)号：US09700252B2

公开(公告)日：2017-07-11

申请号：US13958404

申请日：2013-08-02

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Arnulf Staib ,  Reinhold Mischler ,  Martin Hajnsek ,  Harvey Buck ,  Walter Jernigan

IPC: A61B5/05 ,  A61B5/1486 ,  A61B5/145

CPC classification number: A61B5/14865 ,  A61B5/14532

Abstract: An in vivo amperometric sensor is provided for measuring the concentration of an analyte in a body fluid. The sensor comprises a counter electrode and a working electrode, and the working electrode comprises a sensing layer which is generally water permeable and arranged on a support member adjacent to a contact pad. The sensing layer comprises an immobilized enzyme capable of acting catalytically in the presence of the analyte to cause an electrical signal. The sensing layer has an upper surface facing the body fluid and a lower surface facing away from the body fluid, and the immobilized enzyme is distributed within the sensing layer in such a way that the enzyme concentration in the middle between the upper and lower surfaces is at least as high as on the upper surface of the sensing layer.

公开(公告)号：US20150038814A1

公开(公告)日：2015-02-05

申请号：US13958404

申请日：2013-08-02

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Arnulf Staib ,  Reinhold Mischler ,  Martin Hajnsek ,  Harvey Buck ,  Walter Jernigan

IPC: A61B5/1486

CPC classification number: A61B5/14865 ,  A61B5/14532

Abstract: An in vivo amperometric sensor is provided for measuring the concentration of an analyte in a body fluid. The sensor comprises a counter electrode and a working electrode, and the working electrode comprises a sensing layer which is generally water permeable and arranged on a support member adjacent to a contact pad. The sensing layer comprises an immobilized enzyme capable of acting catalytically in the presence of the analyte to cause an electrical signal. The sensing layer has an upper surface facing the body fluid and a lower surface facing away from the body fluid, and the immobilized enzyme is distributed within the sensing layer in such a way that the enzyme concentration in the middle between the upper and lower surfaces is at least as high as on the upper surface of the sensing layer.