公开(公告)号：US20140251826A1

公开(公告)日：2014-09-11

申请号：US14199003

申请日：2014-03-06

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Karl-Heinz Koelker ,  Arnulf Staib

IPC: G01N27/327

CPC classification number: G01N27/3271 ,  A61B5/1486 ,  A61B5/14865 ,  A61B2562/0209 ,  A61B2562/125 ,  A61B2562/166 ,  C12Q1/004 ,  C12Q1/006 ,  G01N27/3272 ,  G01N33/5438

Abstract: Biosensors and biosensor systems are disclosed that have manganese (III) oxide (Mn2O3)-based electrodes that can attenuate interference of a detection signal resulting from an analyte-relevant reaction caused by undesired reaction of interferents in a sample. Methods are also disclosed for making and using the same.

Abstract translation: 公开了具有氧化锰（III）的电极的生物传感器和生物传感器系统，其可以减弱由样品中干扰物的不希望的反应引起的与分析物相关的反应产生的检测信号的干扰。 还公开了制造和使用该方法的方法。

公开(公告)号：US20170000396A9

公开(公告)日：2017-01-05

申请号：US13958404

申请日：2013-08-02

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Arnulf Staib ,  Reinhold Mischler ,  Martin Hajnsek ,  Harvey Buck ,  Walter Jernigan

IPC: A61B5/1486

CPC classification number: A61B5/14865 ,  A61B5/14532

Abstract: An in vivo amperometric sensor is provided for measuring the concentration of an analyte in a body fluid. The sensor comprises a counter electrode and a working electrode, and the working electrode comprises a sensing layer which is generally water permeable and arranged on a support member adjacent to a contact pad. The sensing layer comprises an immobilized enzyme capable of acting catalytically in the presence of the analyte to cause an electrical signal. The sensing layer has an upper surface facing the body fluid and a lower surface facing away from the body fluid, and the immobilized enzyme is distributed within the sensing layer in such a way that the enzyme concentration in the middle between the upper and lower surfaces is at least as high as on the upper surface of the sensing layer.

Abstract translation: 提供体内电流传感器用于测量体液中分析物的浓度。 传感器包括对电极和工作电极，并且工作电极包括通常为水可渗透并且布置在与接触垫相邻的支撑构件上的感测层。 感测层包含能够在分析物存在下催化作用以产生电信号的固定化酶。 感测层具有面向体液的上表面和背离体液的下表面，并且固定化酶分布在感测层内，使得上下表面中间的酶浓度为 至少与感测层的上表面一样高。

公开(公告)号：US20150005605A1

公开(公告)日：2015-01-01

申请号：US14486402

申请日：2014-09-15

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Arnulf Staib ,  Marcel Thiele ,  Karl-Heinz Koelker ,  Ewald Rieger

IPC: G01N27/327 ,  A61B5/145 ,  A61B5/1486

Abstract: An electrode system is disclosed for measuring a concentration or presence of an analyte under in-vivo conditions, where the electrode system includes at least one electrode with immobilized enzyme molecules and an improved diffusion barrier that controls diffusion of the analyte from body fluid surrounding the electrode system to the enzyme molecules. The diffusion barrier includes a hydrophilic polyurethane or a block copolymer having at least one hydrophilic block and at least one hydrophobic block. The electrode system also can include a spacer membrane that includes a hydrophilic copolymer of acrylic and/or methacrylic monomers.

Abstract translation: 公开了用于在体内条件下测量分析物的浓度或存在的电极系统，其中电极系统包括具有固定化酶分子的至少一个电极和改善的扩散阻挡层，其控制分析物从电极周围的体液的扩散 系统对酶分子。 扩散阻挡层包括具有至少一个亲水嵌段和至少一个疏水嵌段的亲水性聚氨酯或嵌段共聚物。 电极系统还可以包括间隔膜，其包括丙烯酸和/或甲基丙烯酸单体的亲水共聚物。

公开(公告)号：US20140018653A1

公开(公告)日：2014-01-16

申请号：US14027493

申请日：2013-09-16

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Arnulf Staib ,  Marcel Thiele ,  Karl-Heinz Koelker ,  Ewald Rieger

IPC: A61B5/1486

Abstract: Electrode systems are disclosed for measuring the concentration of an analyte under in vivo conditions, where the systems includes an electrode with immobilized enzyme molecules and an improved diffusion barrier that controls diffusion of the analyte from body fluid surrounding the electrode system to the enzyme molecules. Methods of making and using the system also are disclosed.

Abstract translation: 公开了用于在体内条件下测量分析物浓度的电极系统，其中系统包括具有固定化酶分子的电极和改进的扩散屏障，其控制分析物从电极系统周围的体液扩散到酶分子。 公开了制造和使用该系统的方法。

公开(公告)号：US09700252B2

公开(公告)日：2017-07-11

申请号：US13958404

申请日：2013-08-02

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Arnulf Staib ,  Reinhold Mischler ,  Martin Hajnsek ,  Harvey Buck ,  Walter Jernigan

IPC: A61B5/05 ,  A61B5/1486 ,  A61B5/145

CPC classification number: A61B5/14865 ,  A61B5/14532

Abstract: An in vivo amperometric sensor is provided for measuring the concentration of an analyte in a body fluid. The sensor comprises a counter electrode and a working electrode, and the working electrode comprises a sensing layer which is generally water permeable and arranged on a support member adjacent to a contact pad. The sensing layer comprises an immobilized enzyme capable of acting catalytically in the presence of the analyte to cause an electrical signal. The sensing layer has an upper surface facing the body fluid and a lower surface facing away from the body fluid, and the immobilized enzyme is distributed within the sensing layer in such a way that the enzyme concentration in the middle between the upper and lower surfaces is at least as high as on the upper surface of the sensing layer.

公开(公告)号：US20150038814A1

公开(公告)日：2015-02-05

申请号：US13958404

申请日：2013-08-02

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Arnulf Staib ,  Reinhold Mischler ,  Martin Hajnsek ,  Harvey Buck ,  Walter Jernigan

IPC: A61B5/1486

CPC classification number: A61B5/14865 ,  A61B5/14532

Abstract: An in vivo amperometric sensor is provided for measuring the concentration of an analyte in a body fluid. The sensor comprises a counter electrode and a working electrode, and the working electrode comprises a sensing layer which is generally water permeable and arranged on a support member adjacent to a contact pad. The sensing layer comprises an immobilized enzyme capable of acting catalytically in the presence of the analyte to cause an electrical signal. The sensing layer has an upper surface facing the body fluid and a lower surface facing away from the body fluid, and the immobilized enzyme is distributed within the sensing layer in such a way that the enzyme concentration in the middle between the upper and lower surfaces is at least as high as on the upper surface of the sensing layer.