公开(公告)号：US20150046114A1

公开(公告)日：2015-02-12

申请号：US14524633

申请日：2014-10-27

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Thornsten Brueckner ,  Eloisa Lopez-Calle ,  Norbert Oranth ,  Josef Roedl

IPC: G01N33/92 ,  G06F19/18 ,  G01N21/63 ,  G01N33/573 ,  G01N33/68

CPC classification number: G01N33/54393 ,  G01D18/00 ,  G01N21/274 ,  G01N21/63 ,  G01N21/78 ,  G01N21/82 ,  G01N33/52 ,  G01N33/54313 ,  G01N33/54346 ,  G01N33/573 ,  G01N33/6827 ,  G01N33/92 ,  G01N2021/825 ,  G16B20/00 ,  G16H50/30 ,  Y02A90/26

Abstract: A method for determining the amount of a specific analyte by photometric assays, wherein the specific analyte in a sample reacts with an analyte specific reaction partner in a reaction mixture. At least two calibration curves are generated, the first calibration curve recorded at a first wavelength is optimized for low concentrations of the specific analyte thereby maximizing the lower detection limit and, the second calibration curve recorded at a second wavelength is optimized for high concentrations of the specific analyte thereby maximizing the upper detection limit. The optimized lower detection limit and the optimized upper detection limit results in an extended dynamic range.

Abstract translation: 用于通过光度测定法确定特定分析物的量的方法，其中样品中的特定分析物与反应混合物中的分析物特异性反应伴侣反应。 产生至少两个校准曲线，针对低浓度的特定分析物优化以第一波长记录的第一校准曲线，从而使检测下限最大化，并且以第二波长记录的第二校准曲线被优化用于高浓度 特异性分析物，从而最大化上限检测限。 优化的较低检测限和优化的上限检测极限导致扩展的动态范围。