公开(公告)号：US11099180B2

公开(公告)日：2021-08-24

申请号：US16528710

申请日：2019-08-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Ralf Bollhagen ,  Barbara Upmeier ,  Werner Naser ,  Toralf Zarnt

IPC: G01N33/53 ,  G01N33/543 ,  G01N33/576

Abstract: The disclosure concerns a method and kits for measurement of an analyte in a microparticle-based analyte-specific binding assay. In the assay, the microparticles are coated with the first partner of a binding pair, mixing the coated microparticles and at least two analyte-specific binding agents, each conjugated to the second partner of the binding pair, and a sample suspected of containing the analyte. The second partner of the binding pair is bound to each of the analyte-specific binding agents via a linker comprising from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agents to the coated microparticles. The method also entails separating the microparticles having the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.