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1.发明申请公开(公告)号：US20110203927A1
公开(公告)日：2011-08-25
申请号：US13121395
申请日：2009-10-02
IPC分类号： G01N27/447 , G01N33/559 , G01N33/68 , B82Y40/00
CPC分类号： G01N27/44717 , G01N33/54346 , G01N2015/0038 , G01N2015/0693
摘要： The present invention provides methods, apparatuses and kits for determining the presence and the concentration of nanoparticles in a given area, solution or region via cellular uptake and/or adsorption monitored through laboratory equipment. For example, the present invention provides a method of quantifying one or more nanoparticles by incubating a nanoparticle solution comprising one or more nanoparticles with one or more cells; isolating the one or more cells; lysing the one or more cells to release a cell lysate; separating the cell lysate electrophoretically on a gel; digitizing the gel to form a gel image; quantifying the nanoparticle intensity in the gel image; and correlating the nanoparticle intensity to a cell-associated nanoparticle concentration.
摘要翻译： 本发明提供用于通过实验室设备监测的细胞摄取和/或吸附来确定给定区域，溶液或区域中纳米颗粒的存在和浓度的方法，装置和试剂盒。 例如，本发明提供了通过将包含一种或多种纳米颗粒的纳米颗粒溶液与一种或多种细胞孵育来定量一种或多种纳米颗粒的方法; 隔离一个或多个细胞; 裂解一个或多个细胞以释放细胞裂解物; 在凝胶上电泳分离细胞裂解物; 将凝胶数字化以形成凝胶图像; 量化凝胶图像中的纳米颗粒强度; 并将纳米颗粒强度与细胞相关纳米颗粒浓度相关联。
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2.发明授权Method for measuring carbon nanotubes taken-up by a plurality of living cells 有权用于测量由多个活细胞摄取的碳纳米管的方法公开(公告)号：US08632671B2
公开(公告)日：2014-01-21
申请号：US13121395
申请日：2009-10-02
IPC分类号： G01N27/447
CPC分类号： G01N27/44717 , G01N33/54346 , G01N2015/0038 , G01N2015/0693
摘要： The present invention provides methods, apparatuses and kits for determining the presence and the concentration of nanoparticles in a given area, solution or region via cellular uptake and/or adsorption monitored through laboratory equipment. For example, the present invention provides a method of quantifying one or more nanoparticles by incubating a nanoparticle solution comprising one or more nanoparticles with one or more cells; isolating the one or more cells; lysing the one or more cells to release a cell lysate; separating the cell lysate electrophoretically on a gel; digitizing the gel to form a gel image; quantifying the nanoparticle intensity in the gel image; and correlating the nanoparticle intensity to a cell-associated nanoparticle concentration.
摘要翻译： 本发明提供用于通过实验室设备监测的细胞摄取和/或吸附来确定给定区域，溶液或区域中纳米颗粒的存在和浓度的方法，装置和试剂盒。 例如，本发明提供了通过将包含一种或多种纳米颗粒的纳米颗粒溶液与一种或多种细胞孵育来定量一种或多种纳米颗粒的方法; 隔离一个或多个细胞; 裂解一个或多个细胞以释放细胞裂解物; 在凝胶上电泳分离细胞裂解物; 将凝胶数字化以形成凝胶图像; 量化凝胶图像中的纳米颗粒强度; 并将纳米颗粒强度与细胞相关纳米颗粒浓度相关联。
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公开(公告)号：US06487326B1
公开(公告)日：2002-11-26
申请号：US09450156
申请日：1999-11-29
IPC分类号： G02B610
CPC分类号： G01N21/7703
摘要： An apparatus and method is disclosed for detecting an analyte using a fiber optic electrochemical sensor that includes a fiber optic layer, an electrically conductive translucent metallic layer and a light energy absorbing dye layer.
摘要翻译： 公开了一种用于使用包括光纤层，导电半透明金属层和光能吸收染料层的光纤电化学传感器检测分析物的装置和方法。
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4.发明授权Superresolution imaging fiber for subwavelength light energy generation and near-field optical microscopy 失效用于亚波长光能发生和近场光学显微镜的超分辨成像光纤
公开(公告)号：US5633972A
公开(公告)日：1997-05-27
申请号：US564488
申请日：1995-11-29
申请人： David R. Walt , Paul Pantano
发明人： David R. Walt , Paul Pantano
摘要： The present invention provides a superresolution imaging fiber for generating a plurality of subwavelength light energy beams concurrently and for near-field viewing. The imaging fiber comprises a unitary fiber optic array of fixed configuration and dimensions comprising typically from 1,000 to 100,000 optical fiber strands which terminate at one array end as tapered strand end faces limited in size to a range from about 2-1,000 nanometers in diameter. Overlyng these tapered strand end faces is a thin opaque metal coating having a size-limited end aperture ranging from about 2 to less than about 1,000 nanometers in diameter. These size-limited end aperatures provide for the generation of a plurality of discrete subwavelength light beams concurrently.
摘要翻译： 本发明提供了一种用于同时产生多个亚波长光能束并用于近场观看的超分辨率成像光纤。 成像光纤包括具有固定构造和尺寸的单一光纤阵列，其包括通常为1,000至100,000个光纤线束，其在一个阵列端处终止，其中锥形线束端面的尺寸限制在约2-1,000纳米直径的范围内。 超过这些锥形股线端面是薄的不透明金属涂层，其具有尺寸限制的端部孔径在约2至小于约1000纳米的直径。 这些尺寸限制的端部温度同时提供了多个离散的亚波长光束的产生。
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公开(公告)号：US06790672B2
公开(公告)日：2004-09-14
申请号：US10079635
申请日：2002-02-19
IPC分类号： G01N2164
CPC分类号： G01N21/6452 , B01J2219/0045 , B01J2219/00466 , B01J2219/00502 , B01J2219/00545 , B01J2219/00576 , B01J2219/00648 , B01J2219/00702 , B01J2219/0072 , C40B60/14 , G01N21/6428 , G01N21/7703 , G01N2201/0846
摘要： A molecular sieve particle-based analytic chemistry system is disclosed in which populations of encoded molecular sieve particles carrying different chemical functionalities are distributed into wells etched in an optical fiber bundle. The chemical functionalities are encoded on separate shaped molecular sieve particles using luminescent dyes and/or molecular sieve particle shapes and thus, a single sensor array may carry thousands of chemistries. Such encoded molecular sieve particles can provide at least a five-fold enhancement in tunable parameters for increasing the encoding possibilities of high throughput screening assays relative to the present dye-modified polymeric microsphere standard.
摘要翻译： 公开了一种基于分子筛颗粒的分析化学系统，其中携带不同化学官能度的编码分子筛颗粒的群体分布在蚀刻在光纤束中的孔中。 化学功能性使用发光染料和/或分子筛颗粒形状在不同形状的分子筛颗粒上编码，因此，单个传感器阵列可带有数千种化学物质。 这样编码的分子筛颗粒可提供可调参数的至少五倍增强，以增加相对于本发明的染料改性聚合物微球体标准的高通量筛选测定的编码可能性。
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