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公开(公告)号：US20090191581A1
公开(公告)日：2009-07-30
申请号：US12245546
申请日：2008-10-03
申请人： Ronald T. Raines , Stephen M. Fuchs
发明人： Ronald T. Raines , Stephen M. Fuchs
CPC分类号： C12Q1/37 , C07K14/43595 , G01N33/5005
摘要： This invention relates to methods and compositions for designing novel fluorescent proteins, preferably to a green fluorescent proteins (GFP). The engineered GFPs are modified by substituting negatively charged amino acids with positively charged amino acids on the exterior of the protein making the protein cell permeable. The ability of the engineered fluorescent proteins to permeate cells obviates the need for transfections, allowing these novel proteins to be used in numerous biological applications.
摘要翻译： 本发明涉及用于设计新的荧光蛋白，优选绿色荧光蛋白（GFP）的方法和组合物。 通过在蛋白质外部用带正电荷的氨基酸取代带负电荷的氨基酸来修饰工程化的GFP，使得蛋白质细胞是可渗透的。 工程化荧光蛋白渗透细胞的能力消除了转染的需要，允许这些新型蛋白质用于许多生物应用。
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公开(公告)号：US08834918B2
公开(公告)日：2014-09-16
申请号：US12017953
申请日：2008-01-22
CPC分类号： A61K9/7007 , A61K47/645
摘要： A composition for delivery of a molecule into a cell is provided. The composition includes a protein transduction domain that is conjugated to the molecule which is incorporated into a multilayered film. Preferably, the protein transduction domain is a cationic protein transduction domain. More preferably, the cationic protein transduction domain is nonaarginine, and the multilayered film includes polyelectrolyte multilayers. When the composition is presented to a cell, the multilayered film dissolves or erodes in physiological media, and the molecule is delivered into the cell.
摘要翻译： 提供了将分子递送到细胞中的组合物。 该组合物包括与分子结合的蛋白转导结构域，其结合到多层膜中。 优选地，蛋白质转导结构域是阳离子蛋白转导结构域。 更优选地，阳离子蛋白转导结构域是非精氨酸，多层膜包括聚电解质多层。 当将组合物提供给细胞时，多层膜在生理介质中溶解或侵蚀，并将分子递送至细胞中。
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公开(公告)号：US07452973B2
公开(公告)日：2008-11-18
申请号：US11593664
申请日：2006-11-07
申请人： Ronald T. Raines , Stephen M. Fuchs
发明人： Ronald T. Raines , Stephen M. Fuchs
CPC分类号： C12Q1/37 , C07K14/43595 , G01N33/5005
摘要： This invention relates to methods and compositions for designing novel fluorescent proteins, preferably to a green fluorescent proteins (GFP). The engineered GFPs are modified by substituting negatively charged amino acids with positively charged amino acids on the exterior of the protein making the protein cell permeable. The ability of the engineered fluorescent proteins to permeate cells obviates the need for transfections, allowing these novel proteins to be used in numerous biological applications.
摘要翻译： 本发明涉及用于设计新的荧光蛋白，优选绿色荧光蛋白（GFP）的方法和组合物。 通过在蛋白质外部用带正电荷的氨基酸取代带负电荷的氨基酸来修饰工程化的GFP，使得蛋白质细胞是可渗透的。 工程化荧光蛋白渗透细胞的能力消除了转染的需要，允许这些新型蛋白质用于许多生物应用。
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公开(公告)号：US07973132B2
公开(公告)日：2011-07-05
申请号：US12245546
申请日：2008-10-03
申请人： Ronald T. Raines , Stephen M. Fuchs
发明人： Ronald T. Raines , Stephen M. Fuchs
CPC分类号： C12Q1/37 , C07K14/43595 , G01N33/5005
摘要： This invention relates to methods and compositions for designing novel fluorescent proteins, preferably to a green fluorescent proteins (GFP). The engineered GFPs are modified by substituting negatively charged amino acids with positively charged amino acids on the exterior of the protein making the protein cell permeable. The ability of the engineered fluorescent proteins to permeate cells obviates the need for transfections, allowing these novel proteins to be used in numerous biological applications.
摘要翻译： 本发明涉及用于设计新的荧光蛋白，优选绿色荧光蛋白（GFP）的方法和组合物。 通过在蛋白质外部用带正电荷的氨基酸取代带负电荷的氨基酸来修饰工程化的GFP，使得蛋白质细胞是可渗透的。 工程化荧光蛋白渗透细胞的能力消除了转染的需要，允许这些新型蛋白质用于许多生物应用。
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公开(公告)号：US07098016B2
公开(公告)日：2006-08-29
申请号：US10461713
申请日：2003-06-13
IPC分类号： C12N9/22
CPC分类号： C12N9/22
摘要： An enzyme is re-engineered to be a zymogen, an enzyme precursor which is converted into an enzyme by protease cleavage. In the example described here, an RNase A enzyme is converted into a zymogen by adding to the enzyme a bridge of amino acids linking the amino and carboxyl termini of the enzyme. The bridge has built in it a protease cleavage site for a specific protease, for example the protease plasmepsin II, produced by the malaria parasite. Since RNase A can be made cytotoxic, this permits a cytotoxic enzyme to be made in the form of a zymogen that becomes active only when it is acted on by a protease only present in a particular target cell such as a pathogen.
摘要翻译： 酶被重新设计为酶原，酶原，其通过蛋白酶切割转化成酶。 在本文所述的实施例中，通过向酶加入连接酶的氨基和羧基末端的氨基酸桥，将RNase A酶转化为酵素。 该桥已经建立了特定蛋白酶的蛋白酶切割位点，例如由疟原虫产生的蛋白酶等质粒蛋白酶II。 由于RNA酶A可以被制成细胞毒性，因此允许细胞毒素酶以酶原的形式制备，只有当其被存在于特定靶细胞例如病原体中的蛋白酶作用时才变成活性。
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公开(公告)号：US09758569B2
公开(公告)日：2017-09-12
申请号：US11807270
申请日：2007-05-25
CPC分类号： C07K14/78 , C07K5/0821 , C07K14/001
摘要： Novel collagen mimics are disclosed with a tripeptide unit having the formula (Xaa-Yaa-Gly)n, where one of the positions Xaa or Yaa is a bulky, non-electron withdrawing proline derivative. By substituting a proline derivative at either the Xaa or Yaa position in the native collagen helix, the stability of the helix is increased due solely to steric effects relative to prior known collagen-related triple helices. Methods are also disclosed for making the novel collagen mimics.
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公开(公告)号：US08293872B2
公开(公告)日：2012-10-23
申请号：US13243373
申请日：2011-09-23
IPC分类号： C07K1/00
摘要： This invention relates to cytotoxic variants of human ribonuclease 1 (RNase 1) identified through analysis of the interaction between RNase 1 and the human ribonuclease inhibitor (hRI) as defined by the three dimensional (3-D) atomic structure of the RNase1 hRI complex. Also disclosed is the 3-D structure of the hRI•RNase 1 complex and methods for designing the RNase 1 variants.
摘要翻译： 本发明涉及通过分析由RNase1 hRI复合物的三维（3-D）原子结构定义的核糖核酸酶1和人核糖核酸酶抑制剂（hRI）之间的相互作用而鉴定的人核糖核酸酶1（核糖核酸酶1）的细胞毒性变体。 还公开了hRI·RNase 1复合物的3-D结构和用于设计RNA酶1变体的方法。
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公开(公告)号：US07932239B2
公开(公告)日：2011-04-26
申请号：US11412353
申请日：2006-04-27
申请人： Ronald T. Raines , Leonard A. Levin
发明人： Ronald T. Raines , Leonard A. Levin
摘要： Methods and compositions involving a class of boron-protected phenylphosphine agents having increased cell permeability and having improved chemical stability for treating or for preventing neuronal cell death-related diseases or conditions in a human or a non-human animal.
摘要翻译： 涉及一类具有增强的细胞通透性并具有改善的化学稳定性的硼保护的苯基膦试剂的方法和组合物，用于治疗或预防人类或非人动物中的神经元细胞死亡相关疾病或病症。
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9.发明申请STABILIZATION OF THE COLLAGEN TRIPLE HELIX BY O-METHYLATION OF HYDROXYPROLINE RESIDUES 有权通过羟基丙烯酸残基的O-甲基化对胶原三肽螺旋体的稳定化公开(公告)号：US20090264626A1
公开(公告)日：2009-10-22
申请号：US12367374
申请日：2009-02-06
申请人： Ronald T. Raines , Frank W. Kotch
发明人： Ronald T. Raines , Frank W. Kotch
IPC分类号： C07K14/78
CPC分类号： C07K14/78
摘要： This invention relates to a collagen polypeptide comprising a tripeptide motif having the formula (ProYaaGly)n, where Yaa is an O-methylated amino acid residue and “n” is the number of motif repeats. Preferred O-methylated amino acid residues at the Yaa position include (2S,4R)-4-methoxyproline. Other suitable amino acid residues at that position include O-mono or O-di-halogenated methylproline. Also, disclosed is a method of making a synthetic or a semi-synthetic collagen polypeptide molecule having increased stability relative to natural collagen. The strengthened collagen molecules are suitable for use in biomaterials for the medical field or in leather-related products prepared by the tanning industry.
摘要翻译： 本发明涉及包含具有式（ProYaaGly）n的三肽基序的胶原多肽，其中Yaa是O-甲基化氨基酸残基，“n”是基序重复的数目。 在Yaa位置优选的O-甲基化氨基酸残基包括（2S，4R）-4-甲氧基脯氨酸。 该位置上的其它合适的氨基酸残基包括O-单或O-二卤代甲基脯氨酸。 此外，还公开了制备相对于天然胶原蛋白具有增加的稳定性的合成或半合成胶原多肽分子的方法。 强化的胶原分子适用于医疗领域的生物材料或由鞣革行业制备的皮革相关产品。
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公开(公告)号：US07534902B2
公开(公告)日：2009-05-19
申请号：US10988979
申请日：2004-11-15
IPC分类号： C07D311/82 , C07D311/78 , C07D311/88
CPC分类号： C12Q1/34 , C07D493/10 , C09B11/24 , C09B19/00 , G01N33/581 , G01N33/582 , G01N2333/916 , G01N2333/924
摘要： Latent fluorescent compounds, comprising a fluorescent molecule with one or more blocking groups attached and optionally one or more urea-containing groups are provided. The urea-containing group can be used to further attach one or more molecules of interest, such as proteins, peptides or nucleic acids. The blocking group(s) is released from the latent fluorescent compound by reaction with a trigger, forming the fluorescent molecule which can be detected. Also provided herein are methods of using latent fluorescent compounds to detect triggers.
摘要翻译： 提供了包含具有一个或多个连接基团和任选的一个或多个含脲基团的荧光分子的潜在荧光化合物。 含尿素基团可用于进一步连接一种或多种感兴趣的分子，例如蛋白质，肽或核酸。 通过与触发剂反应，阻断基团从潜在荧光化合物释放，形成可被检测的荧光分子。 本文还提供了使用潜伏荧光化合物来检测触发剂的方法。
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