公开(公告)号：US20220252542A1

公开(公告)日：2022-08-11

申请号：US17575551

申请日：2022-01-13

申请人： Roswell Biotechnologies, Inc.

发明人： Barry Merriman ,  Sonal Jain ,  Drew Kennedy ,  Venkatesh Alagarswamy Govindaraj ,  Calvin Gardner ,  Chulmin Choi ,  Namseok Park ,  Richard Dubielzig ,  Le Zheng ,  David Porras ,  Paul Pan

IPC分类号： G01N27/447 ,  B03C5/00

摘要： The disclosed embodiments relate to nanotechnology and to nano-electronics and molecular electronic sensors. In an exemplary embodiment, a nano-sensor having a nanoparticle complex attached at each end to a respective nano-electrode. An exemplary nanoparticle complex includes a biomolecule coupled at each end to a metallic nanoparticle to form a dumbbell-shaped molecular bridge. A method to manufacture single molecule dumbbell nanowires for forming conductive molecular bridges includes the steps of: providing a double-stranded nucleic acid with terminal 3′ thiol modification on both the strands conjugated to a gold (Au) nanoparticle (AuNP) on each end; purifying single biomolecule dumbbells from aggregates using size-exclusion chromatography; imaging the eluted products by electron microscopy to validate formation of single molecule dumbbells; trapping a single molecule dumbbell between a pair of nanoelectrodes on a substrate, the electrodes separated by a nanogap; and measuring the conductivity of a trapped single molecule dumbbell.

公开(公告)号：US20220136993A1

公开(公告)日：2022-05-05

申请号：US17465804

申请日：2021-09-02

申请人： Roswell Biotechnologies, Inc

发明人： Barry Merriman ,  Sonal Jain ,  Drew Kennedy ,  Venkatesh Alagarswamy Govindaraj

IPC分类号： G01N27/327 ,  C01G7/00

摘要： The disclosed embodiments relate to nanotechnology and to nano-electronics and molecular electronic sensors. In an exemplary embodiment, a nano-sensor having a nanoparticle complex attached at each end to a respective nano-electrode. An exemplary nanoparticle complex includes a biomolecule coupled at each end to a metallic nanoparticle to form a dumbbell-shaped molecular bridge. A method to manufacture single molecule dumbbell nanowires for forming conductive molecular bridges includes the steps of: providing a double-stranded nucleic acid with terminal 3′ thiol modification on both the strands conjugated to a gold (Au) nanoparticle (AuNP) on each end; purifying single biomolecule dumbbells from aggregates using size-exclusion chromatography; imaging the eluted products by electron microscopy to validate formation of single molecule dumbbells; trapping a single molecule dumbbell between a pair of nanoelectrodes on a substrate, the electrodes separated by a nanogap; and measuring the conductivity of a trapped single molecule dumbbell.